OF Glucose Deep/Starch Hydrolysis Flashcards
acid production on both open and covered tubes indicate what and what color are they ?
Fermentation and changes the color from green to yellow
Acid production in the open tube (aerobic) and not the oil-covered tube (anaerobic) indicates ?
what color is the open tube and what color is the closed tube?
oxidation because the organism can only metabolize carbohydrate in open condtions
open tube: yellow
closed tube: Green
when both open and closed tubes do not use glucose then you get a negative reaction what color are the tubes?
both green
if there is some blue on top of the green that indicates that there has been an increase in ph
what indicator dye was used in the OF GLUCOSE DEEP TUBE?
bromthymol blue
what color does bromthymol blue turn in the presence of acid?
yellow indicating catabolism of carbohydrates
what color does bromthymol blue turn in the presence of alkaline conditions (basic)? and what about no glucose ?
dark blue color
green
why is mineral oil used?
because the one with mineral is oxygen free
what is a clearing zone in starch hydrolysis?
halo around the colonies formed by exoenzymes
a positive starch test is shown how?
seeing a clearing zone
what is the enzyme and product of starch hydrolysis ?
enzyme: amylase
product: maltose and short chain polysaccharides
what is the reagent used in starch hydrolysis and why?
iodine which reacts with starch
exoenzyme vs endoenzyme?
exoenzyme is secreted outside the cell as opposed to being inside the cell
