Structural stains(endospores, capsule, flagella)

Question 1

structural stains?

Answer 1

used to identify and study structure of bacteria such as endospores, capsules and flagella

Question 2

endospores?

Answer 2

do not metabolize and are resistant to heating, various chemicals and many harsh environment conditions

Question 3

capsule?

Answer 3

when it is round and oval in shape surrounding the bacteria much tougher than slime

Question 4

slime layer

Answer 4

irregular shaped and loosely bound to bacteria

Question 5

glycocalyx

Answer 5

glycoprotein and glycolipid covering that surrounds the cell membrane

Question 6

virulence?

Answer 6

disease-causing ABILITY of some bacteria in which capsules play a role in because when bacteria like streptococcus pneumoniae have capsule it makes it hard for white blood cells to phagocytize the bacteria

Question 7

purpose of endospore stain and (cold method)?

Answer 7

differentiation from bacteria that can produce endospores vs those that dont

time instead of heat

Question 8

the two genre that are identified by endospore technique

Answer 8

bacillus and clostridium

Question 9

what triggers sporulation

Answer 9

stress

when water or essential nutrients are not available

Question 10

clinical signficance of endospore forming bacteria

Answer 10

spore-forming bacteria are resistant to most treatments

Question 11

vegetive cells are stained ?

Answer 11

RED

Question 12

endospores are stained

Answer 12

GREEN

Question 13

what makes the endospores green?

Answer 13

malachite green

Question 14

what does an older culture looks vs newer culture

Answer 14

and older culture contains more spores and cells than a newer one