Lab practicle 1 Flashcards

1
Q

Where do you discard Petri plates and contaminated swabs?

A

Blue Bio Hazard plate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

why must you disinfect your work area before and after lab sessions?

A

to limit the spread of dust-borne organisms as well as destroy any organisms that may have contaminated the work area.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

who was the first researcher to view and describe microorganisms?

A

ANTONI VAN LEEUWENHOEK

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what is a bright field compound microscope?

A

shows dark objects in a bright field

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Field of view?

A

the area seen through a microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Resolution?

A

the ability of a lense to reveal fine detail or two points distinctively apart

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Refractive index?

A

the amount the light bends of the material usually air between the objective lens and the slide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

focal point?

A

That is where an image is formed

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

parfocal?

A

when a subject is in focus with one lens it will be in focus with all the other lenses

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

How do you calculate total magnification?

A

Take the power of the objective and multiply by the power of the eye piece (ocular lense) usually 10X

so OBJECTIVE x OCULAR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

purpose of immersion oil?

A

Reduces refraction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Before using oil immersion you should have your lens in?

A

HIGH POWER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

always focus with ___ first?

A

LOW POWER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Ocular lens

A

remagnifies the image formed by the objective lens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Body tube

A

transmits the image from the objective lens to the ocular lens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

objective lens

A

primary lenses that magnify the specimen

17
Q

mechanical stage

A

holds the microscope slide

18
Q

condenser

A

focuses light through the specimen

19
Q

diaphram

A

controls the amount of light entering the condenser

20
Q

illuminater

A

light source

21
Q

fine focusing knob

A

used for focusing the specimen, turning the knob changes the distant between the objective lens and the specimen