transcription 6 Flashcards
what does self-splicing not require?
a spliceosome
what is the 5’ cap made of and when is it added
7 methyl-guanosine
as soon as the rna emerges from the polymerase
what will attach the cap to the rna
capping enzymes
what is the enzyme that removes the gamma phosphate
rna triphosphatase
what removes the gamma and beta phosphates from gtp
guanylyltransferase
what adds the methyl group to the guanine once it is attached to the mrna
methyltransferase
what provides the binding site for post-transcriptional proteins
the phosphorylated CTD
what are the three things the 5’ cap allow
helps to distinguish mrna
helps mrna be processed
protects from cell degredation
what protects the 5’ cap
cap binding complexes
what do cap-binding complexes act as a marker for
translation
how do viruses cap snatch
they bind to the cap and cleave off our strand and make a new strand
what happens to the 3’ end of mrna and what does this mean
polyadenylated
a string of adenosines are added to the end
what binds to the poly a tail and what does this do
polya binding proteins
stabilise rna
what binds to the conserved aauaa region
4 things
CPSF, poly a pol, CstF, CFI/II
what do the poly a proteins do to the dna
cleave the rna to add the tail
