translation 1 Flashcards

1
Q

what is protein synthesis catalysed by

A

ribozyme

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2
Q

what are the 20 amino acids activated as

A

aminoacyl-trnas

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3
Q

what are the 3 sites on ribosomes

A

EPA

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4
Q

what are the 2 units of rna and what are there sizes

A

small and large
16s and 28s

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5
Q

what defines where the rna stops and stops

A

open reading frame

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6
Q

what does translation initiation require

A

eIFs or IFs

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7
Q

what is the very first step of translation

A

the positioning of the small subunit and aminoacyl-trna

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8
Q

what does translation elongation require

A

efs/eefs

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9
Q

what does translation termination require

A

RFs/ eRFs

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10
Q

what happens when the ribosome reaches the stop codon

A

the ribosome is recycled by RRF/ABCE1

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11
Q

what is contained in the initiation complex

A

fmet-trna, 30s and initiation factors and GTP

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12
Q

what sequence do mrna have on the end that base pairs with rrna and what doe this do

A

shine-Dalgarno
puts the start codon in the p site

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13
Q

what will happen after the s-d is bound

A

50 s unit will bind

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14
Q

what happens to the ribosome before bacterial elongation

A

the initiation factor dissociates, and the 50s binds
making it a 70s

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15
Q

what are the differences between bacteria and eukaryotic mrna

A

a cap and tail
no shine dalagarno sequence
lots of regulation in utr

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16
Q

what is the 5’ cap binding complex made up of and what does it bind to

A

eIF4G, eIF4E, eIF4A and the poly a binding protein
40s subunit