dna replication 4 Flashcards
what does primase synthesise and when will it produce this
a rna primer when attached to helicase
how to we prevent the ssdna from reclosing up again
single-stranded binding protein will bind to prevent closing
what will ssb proteins form and why is this useful
a tetramer meaning the outermost ssb proteins will be easier to remove by the polymerases
what does the primer and the ssb protein trigger and where
the arrival of the pol III holoenzyme and at the 3’ end
what are the three parts of the pol III holoenzyme
pol III core, tau proteins, clamp loader
what does the clamp loader load onto the protein and what then binds to this
beta clamp, the pol III core binds to this
what detaches the beta clamp from the clap loader
atp hydrolysis
what is the beta clamp encoded by and what does it give to the poll II core
dnaN to produce a ring dimer
it gives processivity
what is formed when the pol III catches up to the helicase
replisome
what is a replisome, where is it located and how big is it
combination of the pol III holoenzyme and the primasome
it is at the replication fork
it is around 50 nm
why does the lagging strand start to loop back on itself
as more ssdna is created, it loops back and this allows the primer to work
when will the pol III core bind on the lagging stand
when enough ssdna has emerged
what does helicase and pol III form
trombone model
when will the Okazaki fragment stop being produced
when the poll III core meets the originally added primer which is 5’ to 3’ so it stops as it cannot process dna
what happens when the first Okazaki fragment is made and what happens to poll III core
primase will rebind and add helicase back on and adds on another primer
it will release the strand
