dna replication recap

Question 1

what is the way that ALL organisms carry genetic material

Answer 1

nucleic acid

Question 2

what is cell division for in unicellular and multicellular life

Answer 2

• reproduction
  -growth

Question 3

what is binary fission

Answer 3

doubling of dna

Question 4

how many base pairs do E. coli have

Answer 4

4.6 x 10^6 bps

Question 5

what has the smallest genome

Answer 5

bacteriophage

Question 6

what are the nucleotide monomers

Answer 6

adenosine, cytidine, guanosine, thymidine

Question 7

which bases are purines

Answer 7

adenosine and guanosine

Question 8

which bases are the pyrimidines

Answer 8

cytidine and thymidine

Question 9

how many bases pairs and nucleotides does one turn of the helix have

Answer 9

10.5 bps
~10 nucleotides

Question 10

what are the three key structural features of dna

Answer 10

-double helix
-right handed
-anti- parallel

Question 11

how many h bonds does G-C and A-T have

Answer 11

G-C has 3
A-T has 2

Question 12

why can’t bind you G-T or A-C

Answer 12

as they would be different widths

Question 13

what is semi-conservative replication

Answer 13

when you separate the DNA into two strands and let free nucleotides bind to the template strand

Question 14

which experiment proved semi-conservative replication

Answer 14

meelson and stahl

Question 15

what enzyme is required for PCR

Answer 15

Taq dna polymerase

Question 16

what elements do you need for PCR
hint - 3 elements

Answer 16

dNTPs, template DNA, primers

Question 17

what are the 3 processes of PCR

Answer 17

denaturing, annealing, extending

Question 18

what is broken down to give free energy to the reaction

Answer 18

pyrophosphate