transcription 3 Flashcards
what is the main difference in eukaryotic gene regulation compared to prokaryotic
eukaryotic genes are individually regulated
why are eukaryotic genes individually produced and regulated
because certain genes may be used to make different things for different purposes
what transcribes all genes that encode proteins
rna polymerase 2
how many units does rna polymerase 2 have
12
what can rna polymerase cannot do
it cannot initiate transcription
what do eukaryotic transcription factors do
help rna polymerase bind
what do transcriptional activators do
they help attract rna polymerase 2 to the promotor region and to regulate rate and gene expression
what are ‘other proteins’ responsible for
sorry lol that’s legit what they’ve called them
unwinding chromatin to allow access for the transcription factors
how do proteins recognise the promoter region
the major groove of the double helix
what does the core promoter contain and at what location
TATA box at -30
initiator at +1
where is the enhancer located
-1000
where are the upstream sequence elements located
-100
how does initiation begin in eukaryotes
the TFII position rna pol 2 and separate the dna
which is the first protein to bind and what are the units they contain and what do they do
TFII2
its units are TBP - which recognises and binds to tata bo
TAFs allows other transcription factors to bind
what is the order of proteins binding
lol sorry
TFIID
TFIIA
TFIIB
rna pol
TFIIF
TFIIE
TFIIH
