dna repair 1 Flashcards
what are the two types of dna damage sources
spontaneous and induced
how does a frameshift mutation occur
in a repetitive region, when a nt slips out and reanneals incorrectly and an extra nt is added
what is a tautomeric shift and what will this cause
it is a different form of the base meaning it will bind to a different nt
what is deamination
loss of amino group
what will the deanimation of C, A and G form
c = uracil
a = inosine
g = xanthine
which base cannot be repaired during deanimation
5-methyl-c to t
what is depurination and what does it form
cleavage of the base-sugar bond
abasic site
how do we test how mutagenic something is
ames test
how does the ames test work
we take a salmonella strain that needs histidine and incubate them with the mutagen and without, and if the histidine increases or decreases that is how mutagenic it is
what is an intercalating agent
an agent that inserts itself between bases
what is a base analogue and why is this a problem
an agent that looks like a base
-it is more susceptible to tautomeric shifts
what is an alkylating agent and what can it speed up
an agent that adds a alkyl group to the bases
it can speed up depurination
what is a deanimating agent
agent that removed amino groups
what is an oxidising agent
agent that oxidises bases
which base is most prone to mutation
G
