Topic 7 Flashcards

1
Q

What are the macromolecules (elements and cations) microbes require?

A

C, O, H, N, P, S and Mg2+, Ca2+, Fe2+, K+

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2
Q

What are the micronutrients required for microbes?

A

Co, Cu, Mn, and Zn

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3
Q

What are autotrophs?

A

organisms that fix CO2 into organic molecules

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4
Q

What are phototrophs?

A

organisms that obtain energy through light reactions

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5
Q

What are chemotrophs?

A

Organisms that obtain energy through redox reactions

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6
Q

What are lithotrophs?

A

chemotrophs that use inorganic molecules as a source of electrons

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7
Q

What are organotrophs?

A

chemotrophs that use organic molecules as a source of electrons

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8
Q

What are heterotrophs?

A

organisms that use organic carbon sources from autotrophs

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9
Q

What are diazotrophs?

A

use O2 sensitive nitrogenase enzyme to convert inorganic nitrogen into ammonium so other organisms can use it

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10
Q

What is a complex media and what microbes might it be used for?

A

nutrient rich medium but unknown chemical composition; heterotrophs

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11
Q

What is a minimal defined media and what microbes might it be used for?

A

contains only essential for growth nutrients; autotrophs

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12
Q

What is an enriched media and what microbes might it be used for?

A

a type of complex media in which specific growth factors are added; molicutes

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13
Q

What is a selective media?

A

favours the growth of one organisms over another

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14
Q

What is a differential media?

A

exploits biochemical differences between two microbes growing

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15
Q

What are oligotrophs?

A

grow optimally at low nutrient concentrations

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16
Q

What are the pros of broth and solid medium?

A

Broth - a closed culture that maximizes number of microbes

Solid - open system, can visualize microbes and count colonies

17
Q

What is the generation time and how is it calculated?

A

the time it takes for a population to double; N0 x 2^n

18
Q

What occurs during the stationary phase of cell growth?

A

cell numbers stop rising due to a lack of key nutrients, cells adapt by sporulating or becoming smaller