Topic 5 Flashcards
Light dependent reaction
- uses light energy to make ATP and NADPH
- takes place in the thylakoid membrane
- also called photophosphorylation
Light independent reaction
- uses products of light dependent reaction to make organic compounds
- takes place in the stroma
- called the Calvin cycle
Parts of a chloroplast
- lamellae
- Chloroplast DNA
- grana: stack of thylakoids
- thylakoid: Large SA, site of light dependent reaction
- starch grain: insoluble, no affect on water potential
- stroma: site of independent reaction
Stages of light dependent reaction
- called photophosphorylation
- photosynthetic pigments absorb light energy
- exists an electron that leaves the chlorophyll in P.S.1 (photoionisation)
- electrons move along the electron transport chain
- electron releases energy
- this energy is used to join ADP+Pi
- NADP is reduced to form NADPH
- photolysis of water produces protons, electrons and oxygen
Light independent reaction
Calvin cycle:
- CO2 combines with RuBP catalysed by the enzyme rubisco
- makes 2 molecules of GP
- both GP are reduced to for 2xTP - from 2xNADPH, 2xATP
- most TP is regenerated to form RuBP - using energy from ATP
- some of the TP is converted into useful organic compounds
Limiting factors of photosynthesis
- Light intensity
- CO2 concentration
- temperature
Light intensity as limiting factor
- the higher the light intensity the more energy there is for LDR so the faster the rate of photosynthesis
- light needs to be the right wavelength (looks green because the reflect green but absorb blue and red)
- different pigment absorbs different wavelengths
(Use light at night, green house allows light to pass in)
CO2 concentration as limiting factor
- CO2 is commonly the limiting factor
- CO2 is about 0.04% of atmosphere
- optimum CO2 concentration = 0.4%
- above 0.4% co2 has a negative effect on rate of photosynthesis
(Burn fossil fuels in a greenhouse, increase CO2 concentration)
Temperature as the limiting factor
- photosynthesis is controlled by enzymes
- if you increase temperature you increase the rate of reaction up to the optimum temperature
- beyond the optimum temperature the rate decreases
- high temperature causes stomata to close, low CO2 therefore low Calvin cycle
(Burner increases temp, greenhouse traps warm air, heating/ cooling to maintain optimum temp)
Aerobic respiration
- needs oxygen
- more efficient (more ATP per molecule of glucose)
- complete breakdown of glucose to form CO2, ATP
- slow
- glycolysis, link reaction, Krebs cycle, oxidative phosphorylation
Anaerobic respiration
- doesn’t need oxygen
- less efficient
- incomplete break down of glucose (makes harmful waste products)
- animals and bacteria = lactic acid
- plants and yeast = ethanol
- fast
- glycolysis
Glycolysis
- takes place in cytoplasm
- anaerobic
- net yield is 2ATP, 2NADH
- glucose is phosphorylated by adding ATP (glucose - glucose phosphate - hexose bisphosphate)
- triose phosphate is oxidises to form pyruvate
- pyruvate is actively transported into mitochondria for link reaction
Link reaction
- pyruvate is dehydrogenated and decarboxylated to form acetate
- in matrix of mitochondria
- acetate combines with coenzyme A to form acetyl coenzyme A
- products: per reaction = 1xCO2, 1xNADH/ per glucose = 2xCO2, 2xNADH
Krebs cycle
- matrix of mitochondria
- Acetyl COA joins a 4C compound to form a 6c compound
- coenzyme A is recycled (link reaction)
- 6C to 5C = decarboxylation
- dehydrogenation
- Substrate level phosphorylation = creation of ATP without ATP synthase, phosphate is added to ADP from another molecule
- lipids/ proteins can also be respired aerobically
- products per cycle = 3xNADH, 1xATP, 1xFADH, 2xCO2
Oxidative phosphorylation
- electrons and protons are released from reduced co enzyme = NADH (e-+H++NAD)/ FADH (e-,H++FAD)
- electrons flow along the electron transport chain in a series of redox reactions
- electrons release energy which is used to join ADP+Pi
- oxygen is the final electron acceptor (combines with electrons and protons to form water)
- oxidative phosphorylation makes most of the ATP in aerobic respiration
Anaerobic respiration - glycolysis in mammals and bacteria
Lactate fermentation:
glucose -(ATP-ADP)- glucose phosphate -(ATP-ADP)- hexose biosphosphate - 2xTP -(2ADP+Pi-ATP, NAD-NADH)- 2xPyruvate -(NADH-NAD) 2xLactate
Anaerobic respiration - glycolysis in plants and yeast
Alcoholic fermentation:
Glucose -(ATP-ADP)- Glucose phosphate -(ATP-ADP)- Hexose biosphosphate - 2TP -(2ADP+Pi-ATP, NAD-NADH)- 2Pyruvate -(-CO2)- 2Ethanal -(NADH-NAD)- 2Ethanol
Anaerobic respiration - glycolysis
- takes place in the cytoplasm
- less effeicient produce of ATP than aerobic respiration
- NAD is needed for glycolysis
- NAD is regeerated by reduced pyrivate
- mammals and bacteria = pyruvate - lactate
- yeast and plants = pyruvate - ethanal - ethanol
Why is there less ATP in anaerobic respiration
- products aren’t totally respired
- they still have chemical energy
- no oxygen as the final electron acceptor (no other stages of aerobic)
Why is there more CO2 produced in anaerobic respiration
- need a minimum amount of ATP to do metabolic processes
- only respire anaerobically you need to use more glucose to make necessary amount of ATP
- therefore you make more CO2 and more waste products as a result
- so less efficient
Respiratory substrate reaction
respiratory substrate +O2 > (many reactions catalysed by intra cellular enzymes) > H2O + CO2 + energy
Respiratory substrate reactions
- Carbohydrates = glucose (glycolysis - link reaction - acetyl COA - Krebs cycle - oxidative phosphorylation)
- lipids = triglycerides (acetyl COA - Krebs cycle - oxidative phosphorylation)
- proteins = amino acids (acetyl COA - Krebs cycle - oxidative phosphorylation)
Every cell respires
- respiration is essential to life
- ATP is needed for all metabolic processes
- every cell must respire
- minimum amount of ATP needed to maintain metabolism
- different respiratory substances have different energy values = Lipids > protein > carbs
- proteins only respire when there is no lipids or carbohydrates