TOPIC 2.1 Eukaryotic Cells Flashcards
Magnification of light microscopes
*1500
magnification of electron microscope
*500,000
define resolution
A measure of how close together two objects must be before we see them as one.
what do we call an image of an object as viewed through a microscope
micrograph
resolving power of light microscope
200nm
resolving power of electron microscope
0.5 nm
total magnification
eyepiece lens magnification*objective lens magnification
strengths and Limitations of Light Microscopes
S-
Cheap
can look at live cells with little prep
L-
lower Magnification and resolving power compared to EM
Instead of a beam of light like LM, EM uses a beam of…..
electrons
EM has a higher resolving power because the wavelength of electron beams is …..
Smaller than LM
Bane 2 types of EMs
TEM-transmission electron microscope
SEM- scanning electron microscope (produces 3D images)
Strengths and Limitations of Electron Microscopes
S-
high magnification and resolving power
L-
very expensive
specimens have to be examined in a vacuum so they have to be dead and dry
specialised skills required to prepare samples
the nucleus is surrounded by a double membrane called the…
nuclear envelope
define nucleolus
dense area almost pure DNA and protein, Produces ribosomes and RNA
What are ribosomes made of
ribosomal RNA and protein
what is a eukaryotic cell
genetic material is contained in a membrane bound nucleus
what are vesicles
membrane bags that hold secretions made in cells
what are gated channels
protein channels through the lipid bilayer of a membrane that are open or closed depending on the conditions in the cell
describe the fluid mosaic model
current model of the structure of the cell membrane including floating proteins forming pores, channels and carrier systems in a lipid bilayer
what is exocytosis
energy requiring process by which vesicle fuses with the cell surface membrane so that the contents are released to the outside of the cell