Topic 21

Question 1

Equation to calculate the Resolving power

Answer 1

1/d = sin u/λ

u = angle of the outermost beam

Question 2

What are the Rayleigh’s Criterion?

Answer 2

The point where resoluition is obtained can’t be determined but seems to be where the central maximum of one airy disk falls onto the first minima of the other air disk.

Distance between these two points in the resolution limit/resolving power.

Question 3

Give the Abbe’s Criterion

Answer 3

At least 2 different beams should enter the objective to see any detail in a microscope

Question 4

Equation to calculate the Numerical aperture of a microscope (Abbe’s Law)

Answer 4

1/d = (n sin u)/λ

n sin u = Numerical aperture of the objective

Question 5

Describe the immersion microscope

Answer 5

Drop of oil to increase numerical aperture between the onjective and the object.

Question 6

Describe the UV Microscope

Answer 6

Small wavelengths to improve resolving power.

Cannot be seen with the eye