Topic 11

Question 1

What is sedimentation dependent on?

Answer 1

Radius of the sphere

Question 2

Give a practical use of sedimentation

Answer 2

Observing distance travelled by the substance to determine different substances.

Question 3

What is the use of centrifugation?

Answer 3

Increase the speed of sedimentation

Question 4

Equation to calculate Sedimentation Velocity due to Gravity

Answer 4

f = Frictional coefficient

Question 5

Equation to calculate Sedimentation Velocity due to a Centrifuge

Answer 5

• ω = angular velocity*
• r = Radius*

Question 6

Equation to calculate the Frictional Coefficient

Answer 6

f = kT/D

• k = Boltzman constant*
• T = Temperature*

Question 7

What is ultracentrifugation? What are the types?

Answer 7

A centrifuge with a high angular velocity:

Analytical and Preparative

Question 8

Describe an Analytical Ultracentrifuge

Answer 8

Equipped with an optical device to determine concentration distributions at any time during measurement.

Question 9

Describe a Preparative Ultracentrifuge

Answer 9

Fractionation of contents and measuring of each fraction

Sedimentation Coefficient obtained by different materials absorbing different wavelengths.