TLC Flashcards
1
Q
Stationary Phase
A
The phase that does not move with the sample.
Reverse: Nonpolar
Normal: Polar
2
Q
Mobile Phase
A
Phase that moves with the sample.
Reverse: Polar
Normal: Nonpolar
3
Q
Partitioning Principle
A
Mixture will be separated into two immiscible liquid phases due to solute molecules dividing. (Stationary and Liquid Phase)
This occurs due to difference in relative solubilities of stationary and mobile phases.
4
Q
How to Calculate Retention Factor
A
Distance of Solute Traveled / Distance of Solvent
5
Q
How to Predict Separation will Turn Out
A
By Polarity !
Opposite Polarity of Stationary Phase will travel farther up plate (Mobile Phase)
6
Q
How to Interpret Polarity of Compounds Based on TLC Results
A
Reverse: Higher RT means more polar
Normal: Higher RT means less polar
