Tissue Processing - Overview Flashcards
1
Q
Histological specimens must well preserved.
- How are samples fixed?
- How large or small should samples be? Should they be thick or thin? How do you achieve the ideal state?
- How can we differentiate parts of a sample?
A
- fixation: usually 10% buffered formalin
- be sufficiently thin → microtome → CUT sections 1 - 7 μm (micrometers) thick
- Have contrast → staining of sections
2
Q
What do you use to dehydrate a sample?
A
Alcohol
3
Q
How do you clear a sample?
A
Xylene
4
Q
The paraffin-embedded tissue is sectioned with a ________.
Sections are ___ to ____ micrometers (μm) in thickness (usually ___ μm). This process is followed by ______.
A
microtome, 1 , 7, 5, staining
5
Q
After a sample is sectioned and put on slide, the tissue needs to be _______ in order to see the contrast, etc. Can be done ______ or with _______ _______.
A
stained, manually, automatic stainer