Thin layer chromotography Flashcards
1
Q
What does TLC do?
A
Allows the analysis of small samples through seperation.
2
Q
What occurs in the stationary phase?
A
Commonly a thin metal sheet coated iin alumina or silica. The solute is adsorbed onto this surface and dependant upon the strength of the attraction the samples will travel varying distances.
3
Q
What occurs during the mobile phase?
A
Either a polar or non polar liquid will flow over the surface of the adsorbed solute. Allowing the solute to travel up the sheet.
4
Q
What can be used to identify hard spots?
A
UV light
Iodine vapour
Ninhydrin (Carsigenic)
5
Q
What calculation can be used to work out the rf value?
A
Rf= Distance travelled by component/ Distance travelled by solvent
