Thin Layer Chromatography

Question 1

The principle on which thin layer chromatography is based is that the
a) Different compounds are absorbed on an absorbent to different degrees
b) Different compounds are absorbed on an absorbent to same degrees
c) Different compounds are adsorbed on an adsorbent to different degrees
d) Different compounds are absorbed on an absorbent to same degrees

Answer 1

c) Different compounds are adsorbed on an adsorbent to different degrees

Question 2

The size of a thin layer of adsorbent is about
a) 0.1 mm
b) 0.2 mm
c) 0.3 mm
d) 0.4 mm

Answer 2

b) 0.2 mm

Question 3

The chromaplate or thin layer chromatography plate is made up of
a) Glass
b) Wood
c) Fibre
d) Metal

Answer 3

a) Glass

Question 4

The eluant filled in the closed jar is
a) Mixture of gases
b) Mixture of a liquid and a gas
c) Mixture of solids
d) Mixture of liquids

Answer 4

d) Mixture of liquids

Question 5

Select the incorrect statement from the following options.
a) The spots of colorless compounds are invisible to the eyes
b) These spots can be detected by putting the plate under ultraviolet light
c) These spots can be detected by placing the plate in a covered jar containing iodine crystals
d) None of the mentioned

Answer 5

d) None of the mentioned

Question 6

The relative adsorption of each components of the mixture is expressed in terms of its
a) Acceleration factor
b) Retardation factor
c) Both acceleration and retardation factor
d) None of the mentioned

Answer 6

b) Retardation factor

Question 7

Select the correct statement from the following.
a) Paper chromatography is a type of partition chromatography
b) A special quality paper is used in paper chromatography
c) Chromatography paper contains water trapped in it, which acts as stationary phase
d) All of the mentioned

Answer 7

d) All of the mentioned

Question 8

Amino acids detected by spraying the plate with ninhydrin solution is an example of
a) Column chromatography
b) Thin layer chromatography
c) Paper chromatography
d) Liquid chromatography

Answer 8

c) Paper chromatography

Question 9

Paper chromatography is based on continuous differential partitioning of components of a mixture between stationary and mobile phases.
a) True
b) False

Answer 9

a) True

Question 10

Retardation factor is the ratio of
a) Distance moved by substance from base line to distance moved by the solvent from base line
b) Distance moved by solvent from base line to distance moved by the substance from base line
c) Distance moved by substance from top line to distance moved by the solvent from top line
d) Distance moved by solvent from top line to distance moved by the substance from top line

Answer 10

a) Distance moved by substance from base line to distance moved by the solvent from base line