Operation of Chromatography

Question 1

Which of the following steps takes place after injection of feed in Column chromatography?
a) Detection of components
b) Separation in the column
c) Elution from the column
d) Collection of eluted components

Answer 1

b) Separation in the column

Question 2

Components with a strong attraction to the support move more slowly than components with weak attraction.
a) True
b) False

Answer 2

a) True

Question 3

What happens during the ‘elution from the column’ phase in chromatography?
a) Components with greatest affinity elute first
b) Components with least affinity elute first
c) Components elute in a random manner
d) Components elute according to their concentration in the mixture

Answer 3

b) Components with least affinity elute firs

Question 4

In chromatogram, the position of peaks on the time axis can be used to determine which of the following?
a) Components of the sample
b) Amount of component in the sample
c) Column efficiency
d) Column resolution

Answer 4

a) Components of the sample

Question 5

In chromatogram, the area under the peak can be used to determine which of the following?
a) Components of the sample
b) Amount of component in the sample
c) Column efficiency
d) Column resolution

Answer 5

b) Amount of component in the sample

Question 6

The stationary phase could be a viscous liquid coated over a surface of solid particles.
a) True
b) False

Answer 6

a) True

Question 7

Using Chromatogram as detector in Chromatography, a graph is obtained between ____________ and time.
a) Quantity
b) Density
c) Concentration
d) Specific gravity

Answer 7

c) Concentration

Question 8

In older analytical methods, which of the following methods were used to allow movement of the mobile phase?
a) Pumps
b) Pressure
c) Gravity
d) Blowing air into the column

Answer 8

c) Gravity