Detection System of Liquid Chromatography

Question 1

Bulk property detectors used in liquid chromatography does not respond to which of the following properties?
a) Refractive index
b) Density
c) Properties of solutes
d) Dielectric constant

Answer 1

c) Properties of solutes

Question 2

Which of the following is not a property of a good detector used in liquid chromatography?
a) Good sensitivity
b) Ability to function in the presence of a large background signal
c) Short response time
d) Volume of detector must be large

Answer 2

d) Volume of detector must be large

Question 3

Detector selectivity is more important in Liquid chromatography than in Gas chromatography.
a) True
b) False

Answer 3

a) True

Question 4

Which of the following UV absorbance detectors provide a real time spectrum of the component of interest?
a) Continuous wavelength detector
b) Variable wavelength detector
c) Scanning wavelength detector
d) Fixed wavelength detector

Answer 4

c) Scanning wavelength detector

Question 5

Fluorescence detection is less selective than absorption detection.
a) True
b) False

Answer 5

b) False

Question 6

Which of the following detectors depend on Snell’s law at the interface between the cell wall and the flowing liquid to deflect the light beam?
a) Electrochemical detectors
b) Fluorescence detectors
c) Refractive index detectors
d) Thermal conductivity detectors

Answer 6

c) Refractive index detectors

Question 7

Refractive index detectors used in liquid chromatography are not based on which of the following processes?
a) Interference
b) Refraction
c) Reflection
d) Absorption

Answer 7

d) Absorption

Question 8

Which of the following detectors can be used for detection of amino acids in protein hydrolyzates by introducing the reagent dansylchloride in the sample?
a) Electrochemical detectors
b) Fluorescence detectors
c) Refractive index detectors
d) Thermal conductivity detectors

Answer 8

b) Fluorescence detectors

Question 9

The reference cell is packed with which of the following in the Adsorption detector used in liquid chromatography?
a) Inactive glass beads
b) Porous glass beads
c) Alumina
d) Silica

Answer 9

a) Inactive glass beads

Question 10

10. Which of the following is true about Laser detectors used in liquid chromatography?
    a) Causes thermal distortion has decreased sensitivity
    b) Causes thermal distortion has increased sensitivity
    c) Does not cause thermal distortion, has decreased sensitivity
    d) Does not cause thermal distortion, has increased sensitivity

Answer 10

a) Causes thermal distortion has decreased sensitivity

Question 11

Which of the following detectors used in liquid chromatography is also called micro-adsorption detector?
a) Electrochemical detectors
b) Fluorescence detectors
c) Refractive index detectors
d) Thermal detectors

Answer 11

d) Thermal detectors

Question 12

In UV-Visible detectors, the cells are not made of which of the following materials?
a) Quartz
b) Teflon
c) Silica
d) KELF

Answer 12

c) Silica

Question 13

Which of the following is not a feature of refractive index type detectors?
a) Higher potential sensitivity
b) Low cost
c) High volume flow rates
d) Easy cell accessibility

Answer 13

c) High volume flow rates

Question 14

Which of the following detectors can detect almost any component?
a) Combining two UV detectors
b) Combining RI and UV detectors
c) Mass detector
d) Laser-based detectors

Answer 14

b) Combining RI and UV detectors