Introduction to Chromatography

Question 1

Chromatography is a physical method that is used to separate and analyse __________
a) Simple mixtures
b) Complex mixtures
c) Viscous mixtures
d) Metals

Answer 1

b) Complex mixtures

Question 2

In which type of chromatography, the stationary phase held in a narrow tube and the mobile phase is forced through it under pressure?
a) Column chromatography
b) Planar chromatography
c) Liquid chromatography
d) Gas chromatography

Answer 2

a) Column chromatography

Question 3

In chromatography, the stationary phase can be ___________ supported on a solid.
a) Solid or liquid
b) Liquid or gas
c) Solid only
d) Liquid only

Answer 3

a) Solid or liquid

Question 4

In chromatography, which of the following can the mobile phase be made of?
a) Solid or liquid
b) Liquid or gas
c) Gas only
d) Liquid only

Answer 4

b) Liquid or gas

Question 5

Which of the following cannot be used as an adsorbent in Column adsorption chromatography?
a) Magnesium oxide
b) Silica gel
c) Activated alumina
d) Potassium permanganate

Answer 5

d) Potassium permanganate

Question 6

Which of the following types of chromatography involves the separation of substances in a mixture over a 0.2 mm thick layer of an adsorbent?
a) Gas liquid
b) Column
c) Thin layer
d) Paper

Answer 6

c) Thin layer

Question 7

Chromatography cannot be used to purify volatile substances.
a) True
b) False

Answer 7

b) False

Question 8

In Column chromatography, the stationary phase is made of _________ and the mobile phase is made of _________
a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas

Answer 8

a) Solid, liquid

Question 9

Chromatography cannot be used to separate delicate products.
a) True
b) False

Answer 9

b) False

Question 10

In Thin layer chromatography, the stationary phase is made of _________ and the mobile phase is made of _________
a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas

Answer 10

a) Solid, liquid

Question 11

In which of the following type of paper chromatography does the mobile phase move horizontally over a circular sheet of paper?
a) Ascending paper chromatography
b) Descending paper chromatography
c) Radial paper chromatography
d) Ascending – descending chromatography

Answer 11

c) Radial paper chromatography

Question 12

Liquid chromatography can be performed in which of the following ways?
a) Only in columns
b) Only on plane surfaces
c) Either in columns or on plane surfaces
d) Neither in columns nor on plane surfaces

Answer 12

c) Either in columns or on plane surfaces

Question 13

Gas chromatography can be performed in which of the following ways?
a) Only in columns
b) Only on plane surfaces
c) Either in columns or on plane surfaces
d) Neither in columns nor on plane surfaces

Answer 13

a) Only in columns

Question 14

In Gas-liquid phase chromatography, the stationary phase is composed of _________ and the mobile phase is made of _________
a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas

Answer 14

c) Liquid, gas

Question 15

Which of the following types of chromatography involves the process, where the mobile phase moves through the stationary phase by the influence of gravity or capillary action?
a) Column Chromatography
b) High Pressure Liquid Chromatography
c) Gas Chromatography
d) Planar Chromatography

Answer 15

d) Planar Chromatography

Question 16

Chromatography is the process for identification, purification and separation of components of a mixture on the basis of
a) Difference in their boiling point
b) Difference in their melting point
c) Difference in their affinity for mobile and stationary phase
d) Difference in their solubility

Answer 16

c) Difference in their affinity for mobile and stationary phase

Question 17

Chromatography involves two mutually
a) Immiscible phases
b) Miscible phases
c) Soluble phases
d) None of the mentioned

Answer 17

a) Immiscible phases

Question 18

There is no need of support if packed into a column when the stationary phase is
a) Gas
b) Liquid
c) Solid
d) None of the mentioned

Answer 18

c) Solid

Question 19

Which of the following is not stationary phase?
a) Liquid-liquid chromatography
b) Gas-liquid chromatography
c) Gas-solid chromatography
d) Solid-solid chromatography

Answer 19

d) Solid-solid chromatography

Question 20

Liquid chromatography is a technique for separating
a) Ions that are not dissolved in a solvent
b) Ions that are dissolved in a solvent
c) Ions that are dissolved in a solute
d) All of the mentioned

Answer 20

b) Ions that are dissolved in a solvent

Question 21

In exclusion chromatography, solute molecules are
separated based on
a) Molecular geometry and size
b) Molecular composition
c) Molecular phase
d) Molecular formula

Answer 21

a) Molecular geometry and size

Question 22

The gas-solid chromatography is ______________
chromatography as per basic principle involved.
a) Exclusion
b) Ion-exchange
c) Adsorption
d) Absorption

Answer 22

c) Adsorption

Question 23

A proper solvent that is passed through the column for elution so that separated components can be collected is called
a) Adsorbent
b) Buffer solution
c) Mobile phase
d) None of the mentioned

Answer 23

b) Buffer solution

Question 24

The relative solubility of solute in both the phases determines the
a) Rate of movement of solvent
b) Rate of disappearance of solvent
c) Rate of movement of solute
d) Rate of disappearance of solute

Answer 24

c) Rate of movement of solute

Question 25

If the mobile phase is gas, movement of solute is determined by its
a) Boiling point
b) Melting point
c) Solubility
d) Volatility

Answer 25

d) Volatility