Column Chromatography

Question 1

Column chromatography is based on the principle of
a) Ion-exchange
b) Exclusion principle
c) Differential adsorption
d) Absorption

Answer 1

c) Differential adsorption

Question 2

Arrange the following compounds in order of their increasing adsorption tendencies.
a) Cellulose&raquo_space; starch&raquo_space; calcium carbonate&raquo_space; alumina
b) Cellulose&raquo_space; starch&raquo_space; alumina&raquo_space; charcoal
c) Charcoal&raquo_space; cellulose&raquo_space; alumina&raquo_space; starch
d) Calcium carbonate&raquo_space;; alumina&raquo_space; starch&raquo_space; cellulose

Answer 2

a) Cellulose&raquo_space; starch&raquo_space; calcium carbonate&raquo_space; alumina

Question 3

What is the factor responsible for the separation in
column chromatography?
a) Polarity differences between the solvent
b) Polarity differences between the solute
c) Polarity indifference between the solvent
d) Polarity indifference between the solute

Answer 3

b) Polarity differences between the solute

Question 4

Select the correct statement from the following options.
a) The lesser the polarity of solute, more strongly it will be
adsorbed on a polar surface
b) The greater the polarity of solute, more weakly it will be
adsorbed on a polar surface
c) The greater the polarity of solute, more strongly it will be adsorbed on a polar surface
d) All of the mentioned option

Answer 4

c) The greater the polarity of solute, more strongly it will be adsorbed on a polar surface

Question 5

The correct order of increasing strength of adsorption is
a) Alkanes&raquo_space; Esters&raquo_space; Aldehydes&raquo_space; Phenols&raquo_space; Ketones
b) Aldehydes&raquo_space; Phenols&raquo_space; Ketones&raquo_space; Esters&raquo_space; Alkanes
c) Aldehydes&raquo_space; Ketones&raquo_space; Esters&raquo_space; Alkanes&raquo_space; Phenols
d) Alkanes&raquo_space; Esters&raquo_space; Ketones&raquo_space; Aldehydes&raquo_space; Phenols

Answer 5

d) Alkanes&raquo_space; Esters&raquo_space; Ketones&raquo_space; Aldehydes&raquo_space; Phenols

Question 6

The components of the mixture in column
chromatography are eluted in order of
a) Increasing polarity and decreasing distribution ratio
b) Increasing polarity and increasing distribution ratio
c) Decreasing polarity and increasing distribution ratio
d) Decreasing polarity and decreasing distribution ratio

Answer 6

b) Increasing polarity and increasing distribution ratio

Question 7

The elution power of a solvent is determined by
a) Its overall polarity
b) The polarity of the stationary phase
c) The nature of the sample components
d) All of the mentioned

Answer 7

d) All of the mentioned

Question 8

Which of the following is separated through column chromatography?
a) Chlorophyll and carotenoids
b) Inorganic cations or complexes
c) Sugar derivatives
d) Amino acids formed by hydrolysis of a protein molecule

Answer 8

a) Chlorophyll and carotenoids

Question 9

The mixture of petroleum ether and benzene is used in
the elution ratio of
a) 1 : 2
b) 1 : 5
c) 1 : 9
d) 1 : 12

Answer 9

c) 1 : 9

Question 10

Chloroform fraction is eluted from the column by
passing chloroform through the column which acts as
a) Eluter
b) Eluant
c) Elution
d) None of the mentioned option

Answer 10

b) Eluant