Column Chromatography Flashcards

1
Q

Column chromatography is based on the principle of
a) Ion-exchange
b) Exclusion principle
c) Differential adsorption
d) Absorption

A

c) Differential adsorption

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2
Q

Arrange the following compounds in order of their increasing adsorption tendencies.
a) Cellulose&raquo_space; starch&raquo_space; calcium carbonate&raquo_space; alumina
b) Cellulose&raquo_space; starch&raquo_space; alumina&raquo_space; charcoal
c) Charcoal&raquo_space; cellulose&raquo_space; alumina&raquo_space; starch
d) Calcium carbonate&raquo_space;; alumina&raquo_space; starch&raquo_space; cellulose

A

a) Cellulose&raquo_space; starch&raquo_space; calcium carbonate&raquo_space; alumina

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3
Q

What is the factor responsible for the separation in
column chromatography?
a) Polarity differences between the solvent
b) Polarity differences between the solute
c) Polarity indifference between the solvent
d) Polarity indifference between the solute

A

b) Polarity differences between the solute

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4
Q

Select the correct statement from the following options.
a) The lesser the polarity of solute, more strongly it will be
adsorbed on a polar surface
b) The greater the polarity of solute, more weakly it will be
adsorbed on a polar surface
c) The greater the polarity of solute, more strongly it will be adsorbed on a polar surface
d) All of the mentioned option

A

c) The greater the polarity of solute, more strongly it will be adsorbed on a polar surface

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5
Q

The correct order of increasing strength of adsorption is
a) Alkanes&raquo_space; Esters&raquo_space; Aldehydes&raquo_space; Phenols&raquo_space; Ketones
b) Aldehydes&raquo_space; Phenols&raquo_space; Ketones&raquo_space; Esters&raquo_space; Alkanes
c) Aldehydes&raquo_space; Ketones&raquo_space; Esters&raquo_space; Alkanes&raquo_space; Phenols
d) Alkanes&raquo_space; Esters&raquo_space; Ketones&raquo_space; Aldehydes&raquo_space; Phenols

A

d) Alkanes&raquo_space; Esters&raquo_space; Ketones&raquo_space; Aldehydes&raquo_space; Phenols

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6
Q

The components of the mixture in column
chromatography are eluted in order of
a) Increasing polarity and decreasing distribution ratio
b) Increasing polarity and increasing distribution ratio
c) Decreasing polarity and increasing distribution ratio
d) Decreasing polarity and decreasing distribution ratio

A

b) Increasing polarity and increasing distribution ratio

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7
Q

The elution power of a solvent is determined by
a) Its overall polarity
b) The polarity of the stationary phase
c) The nature of the sample components
d) All of the mentioned

A

d) All of the mentioned

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8
Q

Which of the following is separated through column chromatography?
a) Chlorophyll and carotenoids
b) Inorganic cations or complexes
c) Sugar derivatives
d) Amino acids formed by hydrolysis of a protein molecule

A

a) Chlorophyll and carotenoids

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9
Q

The mixture of petroleum ether and benzene is used in
the elution ratio of
a) 1 : 2
b) 1 : 5
c) 1 : 9
d) 1 : 12

A

c) 1 : 9

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10
Q

Chloroform fraction is eluted from the column by
passing chloroform through the column which acts as
a) Eluter
b) Eluant
c) Elution
d) None of the mentioned option

A

b) Eluant

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