Amino Acid Analyzer

Question 1

What is the drawback that occurs in using ion exchange chromatography on sulphonated polystyrene resin and colourimetry for amino-acid analysis?
a) Less accuracy
b) Low resolution
c) Inconvenient to handle many individual samples
d) Slow in operation

Answer 1

c) Inconvenient to handle many individual samples

Question 2

Which one of the following methods is the most suitable for amino-acid analysis?
a) Gas chromatography
b) Ion exchange chromatography
c) Paper electrophoresis
d) Resin column chromatography

Answer 2

d) Resin column chromatography

Question 3

Which of the following colour reagents are used in Resin column chromatography?
a) Marquis reagent
b) Benedict reagent
c) Ninhydrin
d) Nessler’s reagent

Answer 3

d) Nessler’s reagent

Question 4

Which of the following amino-acids is measured at a wavelength of 440nm using photometric systems?
a) Proline
b) Alanine
c) Glutamine
d) Valine

Answer 4

a) Proline

Question 5

In Automatic amino-acid analyzer, the sample containing ___________ of each amino compound is introduced at the top of the ion exchange column.
a) 1 to 10 µmoles
b) 1 to 10 moles
c) 0.05 to 2 moles
d) 0.05 to 2 µmoles

Answer 5

d) 0.05 to 2 µmoles

Question 6

Which of the following cannot be analysed using resin column chromatography?
a) Peptides
b) Amines
c) Amino compounds
d) Components which are ninhydrin negative

Answer 6

d) Components which are ninhydrin negative

Question 7

Trapped air in pumps of Automatic amino-acid analyzer is eliminated using which of the following ways?
a) Suction pump
b) De-aerator
c) Overcome naturally by pump pressure
d) Using pulse damper

Answer 7

b) De-aerator

Question 8

Amino-acids are distinguishable from each other only by one or two atoms in their structure.
a) True
b) False

Answer 8

a) True

Question 9

What is the drawback that occurs in using gas chromatography for amino-acid analysis?
a) Costly
b) Cannot be used for proteins or polypeptides
c) Slow process
d) Complex in operation

Answer 9

b) Cannot be used for proteins or polypeptides

Question 10

Resin column chromatography does not involve breaking of bonds in amino-acids.
a) True
b) False

Answer 10

b) False