therio final cats and dogs Flashcards
gold standard to describe canine phase of estrus
endocrinology
bitch behavior
flags tail to right.
bitch cytology
used to determind her place in cycle. testing bioassay of estrogen presence.
cornification
progression of epithelial cell death from tissue thickening due to estrogen. look like corn-flake like, polygonal shaped. non cornified are circular, deeply stained nucleus.
estrus cytology
in estrus, superficial pyknotic cells and intermediate cells and anuclear squamous cornflakes. in diestrus, PMNs. not normal to see during estrus. PMNs clear out foreign material diestrus is abrupt change to non cornified material. high progesterone. time parturition now.
progesterone
estimates l4 peak and time of ovulation. differs in other species becasue no p4 prior to ovulation.
bitch anatomy
abnormalities at junction of urethra and vagina like strictures or remnany hymens. pre-breeding exam done in estrus- on digital exam, feel crvix due to shape of tract. vaginoscopy will show pink, billowy cervix at proestrus and flat, loose crenated at estrus
p4 levels
2-2.9= LH peak
4-10 = ovulation
>10= already ovulated.
breed bitch at 4 and 6 days after LH peak.
2-2-2-2 rule
LH peak at 2-2.9 days. (day 0) ovulation 2 days later. breed two days later (day 4). breed another 2 days later
LH canine
LH peak. abrupt and short. assumed 24 hours, can be less. occasionally 48. ovulation 24-48h later. used daily for very precise timing of LH peak and ovulation. useful with frozen semen because short window of time. used with p4 testing
canine semen usages
fresh-immediately. cooled- 48hrs. frozen- long term, but decreased longevity
bitch wheelbarrow
normal quality fresh or cooled semen. lift bitch’s hindlimbs to allow gravity to facilitate movement of semen down tract.
endoscopic transcervical insemination TCI
intrauterine deposition. small amount needed because of close proximity to fert site. good for fresh/cooled/frozen. surgical is controversial. norwegian catheter for TCI without endoscope
insemination dose
250 million PMMN. for vaginal AI. fresh or chilled. or 100 million PMMN for intrauterine AI. poor quality, frozen, lower doses
gestation length
65d from LH peak, 57d from cytological diestrus. could be up to 72 days from random breeding. body temperature likely fall to 99 day before parturition
bitch’s exam
early- PE, vaginal cytology, digital exam, vaginoscopy, brucellosis screen, HWP, p4 measurements. every 2-3 days recheck p4 levels and cytology. not miss LH surge and estrus. p4 confirm ovulation. check cytology every day for diestrus
feline general
felines are induced ovulators. LH surge is stimulated by tom’s penile spines. ovulation about 24-48 hours later.