The Microscopy

Question 1

earliest form of microscope

Answer 1

crude one; relies on simple lenses and a source of light (usually the natural light of the day)

Question 2

microscope today can produce ______

Answer 2

3D images, contrasts between parts and a higher magnification and resolution

Question 3

two (2) principles of the microscope

Answer 3

1. Magnification
2. Resolution

Question 4

this principle of the microscope is its ability to enlarge an object placed in the field of view

Answer 4

magnification

Question 5

this principle of the microscope is the ability to clarify an object to distinguish parts from one another

Answer 5

resolution

Question 6

magnification and resolution are important to defining the ______

Answer 6

structures of cells, tissues, and microorganisms

Question 7

two (2) classification in which microscope can be classified

Answer 7

based on the:
1. number of lenses
2. source of illumination

Question 8

two (2) microscope classifications based on the number of lenses

Answer 8

1. simple microscope
2. compound microscope

Question 9

two (2) microscope classification based on the source of illumination

Answer 9

1. light microscope
2. electron microscope

Question 10

this classification of microscope makes use of a single lens

Answer 10

simple microscope

Question 11

classification of microscope that makes use of light

Answer 11

light microscope

Question 12

classification of microscope that makes use of beams of electrons and with higher magnification

Answer 12

electron microscope

Question 13

classification of microscope that makes use of two lenses

Answer 13

compound microscope

Question 14

electron microscopes may be either:

Answer 14

1. transmission electron microscope (TEM)
2. scanning electron microscope (SEM)

Question 15

electron microscope that is used to make 3D images of the specimen’s surface

Answer 15

scanning electron microscope

Question 16

electron microscope that produces a 2D image of an ultrathin section by capturing electrons that have passed through the specimen

Answer 16

Transmission electron microscope

Question 17

electron microscope most useful in investigating a cell’s interior

Answer 17

TEM

Question 18

what is done for the specimens under observation to be better viewed?

Answer 18

staining

Question 19

microscope which allows a clear view of a specimen even without staining

Answer 19

phase contrast microscope

Question 20

microscopy wherein fluorescent dyes are used so that, when illuminated by ultraviolet dyes, would fluoresce

Answer 20

fluorescent microscopy

Question 21

microscopy useful in tracking certain signals, genes, and structures of the body

Answer 21

fluorescent microscopoy

Question 22

the ability of microscopes to magnify and resolve objects are made possible by this part:

Answer 22

magnifying parts

Question 23

part that functions for enlargement and resolution of objects

Answer 23

magnifying parts

Question 24

total magnification =

Answer 24

objective magnification x eyepiece magnification

Question 25

magnifying power of the eyepiece

Answer 25

10x

Question 26

five (5) tips you can follow when using the microscope in the laboratory

Answer 26

1. Place the microscope on a level surface 2. Check the parts before using them 3. Handle the knobs and objectives carefully 4. Maximize the use of the mirror 5. Always open both eyes when looking at the specimen

Question 27

why should one check the parts first before using the microscope?

Answer 27

there might be some parts that are missing or broken

Question 28

why should the knobs and objectives be handled carefully?

Answer 28

in order to not loosen them and also to avoid scratching their surfaces

Question 29

why should we maximize the use of the mirror?

Answer 29

this will illuminate the specimen well

Question 30

why should both eyes be open while looking at the specimen?

Answer 30

to prevent your eyes from being strained

Question 31

this microscope is a practical and convenient tool in any Biology laboratory; it uses the visible spectrum for magnification of objects as minute as less than a millimeter in dimension, up to a small fraction of a micron

Answer 31

light compound microscope

Question 32

resolution of a light compound microscope

Answer 32

0.02 microns

Question 33

most commonly used microscope in the laboratory, passing light rays through the specimen, and bringing them to focus using system lenses

Answer 33

bright-field microscope

Question 34

two (2) parts of a light compound microscope

Answer 34

1. optical parts 2. mechanical parts

Question 35

four (4) parts of the microscope under the optical part

Answer 35

1. ocular lens 2. objective lenses 3. abbe condenser lens 4. mirrror

Question 36

ten (10) mechanical parts of the microscope

Answer 36

1. draw tube 2. dust shield 3. revolving nosepiece 4. stage 5. iris diaphragm 6. inclination joint 7. body 8. handle 9. base 10. course and fine wheels

Question 37

part through which the object is viewed

Answer 37

ocular lens

Question 38

used to collect light and has two kinds

Answer 38

mirror

Question 39

4 kinds of objective lenses

Answer 39

1. Scanner 2. LPO 3. HPO 4. Oil Immersion

Question 40

a sub-stage lens that concentrates the light rays that pass through the mounted specimen

Answer 40

abbe condenser lens

Question 41

enumerate the magnification power of the objective lenses

Answer 41

1. scanner - 4x 2. LPO - 10x 3. HPO - 40x 4. Oil immersion - 100x

Question 42

2 kinds of mirror:

Answer 42

1. plane mirror 2. concave mirror

Question 43

this mirror is used with the natural light

Answer 43

plane mirror

Question 44

this mirror is preferred when working with artificial or dim light

Answer 44

concave mirror

Question 45

used for holding the microscope

Answer 45

handle

Question 46

while the hand is on the handle to hold the microscope, the other hand of the user should support its ______

Answer 46

base

Question 47

it is rotated to shift from one objective lens to another

Answer 47

resolving nosepiece

Question 48

a substage centrally attached circular contraption that has overlapping strips of metal arranged akin to those of a folding fan operated with a lever to open or close; serves to regulate the amount of light entering the condenser lens

Answer 48

iris diaphragm

Question 49

pairs of big and small knobs positioned on each side of the body of the microscope

Answer 49

course and fine wheels

Question 50

it adjusts the distance of the eyepiece from the objective lens

Answer 50

draw tube

Question 51

it is a round or forked bottom that must be laid flat on the table; should be supported during the transport of the microscope to and from the stockroom

Answer 51

base

Question 52

a piece of metal disk fastened above the revolving nosepiece

Answer 52

dust shield

Question 53

found only in the early model, but it is conveniently used for tilting the microscope when the observer is a little tired to stoop down

Answer 53

inclination joint

Question 54

quadrangular platform with a hole at the center, is where the specimen is mounted

Answer 54

stage

Question 55

straight tube where the ocular and objective lenses are attached

Answer 55

body

Question 56

a larger microscope that can occupy a substantial part of a laboratory room

Answer 56

electron microscope

Question 57

magnification of an electron microscope can be as high as _________

Answer 57

1 milliion times

Question 58

limit of resolution of an electron microscope

Answer 58

0.2 nm

Question 59

instead of magnifying glass lenses, electron microscope uses ______ for magnification

Answer 59

series of electromagnetic fields

Question 60

electron microscope that can resolve the fine details of a cell in the interior

Answer 60

transmission electron microscope

Question 61

electron microscope that can resolve the details of the surface structure of the cell

Answer 61

scanning electron microscope

Question 62

type of bright field microscope that is usually of a low magnification; used mainly for dissection of tiny objects, providing them a 3D image

Answer 62

stereoscopic microscope

Question 63

type of optical microscope which uses a camera that is able to project an object placed on the stage for examination in a monitor; best used to examine histological details of tissues or the size and arrangement of bacterial cells

Answer 63

digital microscope

Question 64

microscope that uses a pinhole, where a fine beam of light (laser beam) is made to pass through the specimen, eliminating out of focus light rays

Answer 64

confocal microscope

Question 65

other term used to refer to confocal microscope

Answer 65

confocal laser scanning microscope