Testing/laboratory/Phlebotomy Flashcards
Random urine
Patient urinated in a clean, nonsterile container.
Used for screening purposes
First morning urine
Patient collects the first specimen of the morning in a clean container, it is more concentrated.
Used for pregnancy testing, or when other analytes (protein, nitrites) need to be evaluated.
Clean-catch midstream
Pt performs perineal cleaning using wipes, beings to urinate, then collects the specimen midstream in a sterile cup.
Used for cultures or when a noncontaminated specimen is desired.
24-hour urine specimen
Uses a container with preservatives. Pt discards first morning urine and collects for the next 24 hrs, including the first void of the second day.
Type of collection that is important in quantitative analysis.
Catheterized collection
Inserts a catheter when a sterile urine sample is needed and the pt is unable to provide a sample.
Hemoglobin (test values)
Males: 13 to 18 g/dL
Females: 12 to 16 g/dL
Hematocrit (test values)
Male: 42% to 52%
Female: 36% to 48%
Fasting blood glucose (test values)
Male/Female: 60 to 110 mg/dL
Total cholesterol (test values)
Male/Female: less than 200mg/dL
Pt should not eat 12 hrs prior to collecting blood sample
Low-density lipoprotein (test values)
Male/Female: less than 130mg/dL
High-density lipoprotein (test values)
Greater than 40mg/dL
Triglycerides (test values)
Male/Female: less than 150mg/dL
Erythrocyte sedimentation rate (test values)
Male: 0 to 20 mm/hr
Female: 0 to 30 mm/hr
International normalized ratio (INR) (test values)
Male/Female: 0.8 to 1.2
Prothrombin time (test values)
Male/Female: 10.4 to 15.7 seconds
Urine pH (test values)
Male/Female: 4.5 to 8
Urine specific gravity (test values)
Male/Female: 1.005 to 1.030
Urine urobilinogen (test values)
Male/Female: 0.1 to 1
Urine-All other values (test values)
Male/Female: negative
Hyperopia
Difficulty seeing things close up, farsightedness
Myopia
Difficulty seeing things far away; nearsightedness
Presbyopia
Gradual, age-related loss of the eyes’ ability to focus actively on nearby objects
When collecting blood cultures, what type of swabs need to be used?
Povidone-iodine or Chlorhexidine Gluconate swabs
Median cubital vein (phlebotomy)
Located in the center of the antecubital space, most common vein used
Cephalic vein (phlebotomy)
Located in the lateral antecubital space (thumb side)
Basilic vein (phlebotomy)
Vein located in the medial antecubital space; superficial to the brachial artery. Avoid if possible because of a nerve. (Pinky side)
What does inverting mean?
Tilting the tube upside down and returning it to its upright position 3 to 10 times depending on the manufacturer instructions.
Order of draw for microcapillary tubes
- Blood gases
- EDTA tubes
- Other additive tubes
- Serum tubes
Potassium (test values)
Male/Female: 3.5 to 5.1 mEq/L
Sodium (test values)
Male/Female: 135 to 145 mEq/L
Red blood cell count (test values)
Male: 4.7 to 6.1/mm³
Female: 4.2 to 5.4/mm³
Platelets (test values)
Male/Female: 130 to 400/mm³
Serous (wound drainage)
Clear, thin, pale yellow or transparent; may be present in a healthy, healing wound
Serosanguineous (wound drainage)
Containing blood and serum; may also be present in a healthy, healing wound
Sanguineous (wound drainage)
Primarily blood
Purulent (wound drainage)
Thick, white/yellow/brown, and pus-like; may be indicative of infection. Should be cultured.
3 questions for venipuncture?
- Arm preference
- Allergy to latex
- Hx of fainting - if so supine
What does reconstitute mean?
Mixing a powered medication with a liquid medication
Subcutaneous (injection)
45⁰ angle
5/8 needle
23-25 gauge
1mL or less - no more!
Intradermal (injection)
10-15⁰ angle
3/8 needle
25-27 gauge
.1 mL
Intramuscular (injection)
90⁰ angle
1”-3” needle
18-23 gauge
1mL - 3mL
Shaking a blood collection tube causes what?
Hemolysis - rupture or destruction of red blood cells
What is the order of draw for collection tubes?
- Blood cultures
- Light blue (anticoagulant)
- Serum tubes
- Additive tubes
Blood cultures are drawn for what?
To detect the presence of bacteria or fungi in the blood
Blood culture vials (big) & tube (small) colors?
Culture vials: blue, purple, pink
Culture tubes: yellow & black
Blue, purple, & pink culture vials contain? (big bottles)
Culture contains enriched soybean-casin broth with CO²
Yellow & black culture tubes contain? (Small)
Yellow tubes: nutrient solution
Black tubes: blood lysis solution
2 light blue tubes are drawn for?
Drawn before serum tubes to prevent contamination with clot activator
For coagulation studies
Serum tubes
Coated on the inside wall with clot activator, because the surface of the plastic tube does not promote coagulation.
Serum tube colors?
Royal blue (non-additive)
Red
Red with yellow ring on top (SST)
Royal blue serum (non-additive) tubes?
Are metal-free and mainly used for toxicology, trace metal analysis and nutritional studies
Red serum tubes?
Mainly used for serology and chemistry test. Also blood banks
(SST) serum separator tubes? Red with yellow ring
Contains a gel which separates the cells from the serum when the tube is centrifuged.
Pink additive tube?
Testing in blood bank. Blood typing, crossmatching, and antibody screens
Black additive tube?
Black Excyte ESR (erythrocyte sedimentation rate) containing sodium citrate.
Research kits?
Collected after all diagnostic labs have been obtained
FSH (test tubes)
Follicle stimulating hormone
SST, Red
TSH (test tubes)
Thyroid stimulating hormone
SST, Red
PTT (test tubes)
Partial Thromboplastin Time
Light blue
ABG (test tubes)
Arterial blood gases
SST, Red
CBC (test tubes)
Complete Blood Count
Lavender
GC/CT (test tubes)
Chlamydia/Gonorrhea
SST, Red
HGBA1C (test tubes)
Hemoglobin glucose testing
Lavender
VitB12 (test tubes)
Vitamin B12
SST, Red
HSV (test tubes)
Herpes Simplex Virus
SST, Red
HPYLOR (test tubes)
Helicobacter Pylori
SST, Red
LDL (test tubes)
Low density lipoprotein
SST, Red, Lavender
HIV (test tubes)
Human immunodeficiency virus
SST, Red
BMP (test tubes)
Basic Metabolic Panel
SST
PSA (test tubes)
Prostate Specific Antigen
SST, Red
TIBC (test tubes)
Total iron binding capacity
SST, Green
LFT (test tubes)
Liver function Testing
SST, Red
HBV (test tubes)
Hepatitis B Virus
SST, Yellow, Lavender
Rh (test tubes)
Rhesus factor
SST, Red
Blood cultures (test tubes)
Yellow
Triple screen (test tubes)
Panel for AFP, hCG, Estrogen
SST, Red
BUN (test tubes)
Blood, Urea, Nitrogen
SST, Red
ABO (test tubes)
Blood typing
SST, Red
Hgb (test tubes)
Hemoglobin
Lavender, Green
CK (test tubes)
Creatine kinase, total
SST, Green
Testo (test tubes)
Testosterone
SST, Red
GTT (test tubes)
Glucose tolerance test
Gray
VDRL (test tubes)
Venereal disease research
SST, Red
EBV (test tubes)
Epstein-Barr virus
SST, Red
LH (test tubes)
Lutenizing hormone
SST, Red
Hct (test tubes)
Hematocrit
Lavender
MERC (test tubes)
Mercury
Royal blue
HDL (test tubes)
High density lipoprotein
SST, Green, Lavender
K (test tubes)
Potassium
Green
DIGOX (test tubes)
Digoxin
SST, Red
PT (test tubes)
Prothrombin
Light Blue
Na (test tubes)
Sodium
Green
VitD (test tubes)
Vitamin D
SST, Red, Lavender
Meth (test tubes)
Methadone
Red, Lavender, Green
Lead (test tubes)
Lead
Royal blue
HCV (test tubes)
Hepatitis C Virus
SST, Red, Lavender
Sickle cell (test tubes)
Lavender
ESR (test tubes)
Erythrocyte sedimentation rate
Lavender
RA (test tubes)
Rheumatoid arthritis
Red, Lavender
CMP (test tubes)
Complete metabolic panel
SST
FBS (test tubes)
Fasting blood sugar
Gray
Chol (test tubes)
Cholesterol
SST, Lavender
BUN (test values)
Male/Female: 7 to 25 mg/dL
What types of tests require the application of the chain of custody guidelines?
Drug screens
Forensic studies
Blood alcohol levels
Parentage testing
What are the success maneuvers for spirometry testing?
Quick and forceful start
Fast and steady flow with no variability
Blow for minimum 6 seconds
No coughing during test