Test 2 MABs Flashcards
1
Q
structure of antibody
A
- 2 heavy chains: 50kD
- 2 light chains: 25kD
- connected via disulfide bond @ hinge region
2
Q
CDR
A
- hyper-variable region
- antigen binding site
3
Q
Which cells make antibodies?
A
B-cells
4
Q
murine Ab
A
- complete mouse
- omab
5
Q
chimeric Ab
A
- mouse FAB, human Fc portion
- ximab
6
Q
humanized Ab
A
- mainly human; some mouse
- zumab
7
Q
fully humanized Ab
A
- completely human
- umab
8
Q
What was the effect of the humanized Ab?
A
adverse reactions went from 50% to 0.1-34%
9
Q
papain
A
digest Ab into Fab and Fc portions
10
Q
pepsin
A
digest Ab into (Fab)2 and Fc portion into small fragments
11
Q
scFv
A
- variable regions joined together via peptide linkage
- smallest in Ab production that we have reached
12
Q
Where are Bcells located?
A
spleen
13
Q
Hybridoma
A
B cells fused with myeloma cells to produce antibodies indefinitely
14
Q
production of MAb
A
- in vitro manipulation
- introduction into plasmid
- transfer into myeloma cell
- expand growth into tissue culture or mouse for production
15
Q
Results of interaction of mAb with a cell receptor
A
- Direct modulation of target antigen
- Cell damage
