Test 2 Gram (-) Bacteria Flashcards
All gram negative bacteria have this endotoxin
Lipid A Endotoxin
Enterobacteriaceae include which 3 coliform (lactose + )bacterias?
- E.coli*
- Klebsiella*
- Enterobacter*
Vitamin K production is a benefit of this family of residential bacteria
Enterobacteriaceae
These species of bacteria are the
most common nosocomial infections/ Health care associated infections in ICU patients and effect the
Blood stream, urinary tract, respiratory tract
- Klebsiella*
- Enterobacter*
- Proteus*
The complete antigenic formulation of an organism
Serotype
O: O antigen
H: Flagella
F: Fimbriae
K: Capsule
detection of a specific toxic activity, virulence factor or virulence gene
Virotyping
How are strains of *E. coli *identified?
Serotyping
Capsule
Fimbrial adhesins
Non-fimbrial adhesins
Endotoxins
are all _______ virulence factors
and vary by strain
cell-associated
Enterotoxins
Cytotoxins
Siderophores
are all ______ virulence factors
and vary by strain
extracellular
the most frequent bacterium isolated from clinical samples in human and veterinary medicine
E.coli
What causes Edema Disease in Weaned Piglets?
Shigatoxigenic E. coli
What is the mechanism of action of
Shigatoxigenic E. coli
in weaned piglets?
Inhibit protein synthesis by interacting with 60s ribosmes
How do you Dx an enteric disease?
With a FECAL sample
In E. coli, edema markedly expands the colonic _____ ______,
separating glands.
Even at low magnification, the _____ ______ ______ of colonic epithelium
is prominently basophilic
In E. coli, edema markedly expands the colonic lamina propria,
separating glands.
Even at low magnification, the apical brush border of colonic epithelium
is prominently basophilic
Ultrastructurally, _____
attach to the microvillar border with formation of characteristic
“cups and pedestals”
E.coli
Which antimicrobials are *E. coli *resistant to?
Penicillin
Trimethoprim
Tetracycline
Aminoglycosides
Fluoroquinolones
J5 strains are used in the vaccine for this disease caused by E. coli
Coliform mastitis
The most common bacterial isolate from non-human primates with pneumonia is
Klebsiella pneumoniae
This *Klebsiella *related illness is commonly seen in
foals that have failed to acquire passive transfer via colostrum
Naval Ill
17-year-old male rhesus macaque the animal presented acutely
with coughing and hemoptysis and was sedated for evaluation.
The animal was in thin body condition with pale mucous membranes.
Lung sounds were judged to be harsh but heart sounds were normal. Radiographs revealed consolidation of the left lung field and aerophagia of the esophagus and stomach.
Within the cranial left thoracic cavity, there was a small amount of thin, serosanguineous fluid.
The left lung lobes were consolidated and diffusely and firmly adhered to the pleural wall.
Inflammatory nodules efface 50% of the lung. Inflammatory exudate fills alveoli and airways within these areas; numerous vessels contain large fibrin clots
Alveoli contain abundant viable and degenerate neutrophils, foamy macrophages, and innumerable intra- and extracellular 2-3 µm bacilli that are separated and surrounded by a clear capsule
What is the disease?
*Klebsiella pneumoniae. *
capsular serotypes (K1 and K2) that carry genes MagA (mucoviscosity-associated gene/K1 specific capsular polymerase gene) and
rmpA (regulator of mucoid phenotype)
which make this bacteria more invasive and more resistant to phagocytosis.
Klebsiella pneumoniae
• In humans, HMV infection (a hypomucoviscous variant of Klebsiella pneumoniae)
is unusual in that it infects______ ________and causes
liver abscesses, pneumonia, meningitis, and endophthalmitis.
healthy individuals
What is the infectious dose of Shigella?
LOW!
10 -100 organisms only
In Shigella
Ability to enter epithelial cells and cause disease is mediated by chromosomal genes and virulence genes
coded on a large ___ ____ (220 kb) and Type 3 Secretion System
Virulence Plasmid
