TCA Flashcards
NAD+ is derived from?
Vitamin Niacin (3)
FAD is derived from?
Vitamin Riboflavin (2)
Coenzyme A (CoA) is the condensation product of ___, ___, and ___.
Cysteine, B5 (Pantothenic acid), and ATP
Acetyl group is bond to Mercaptoethylamine residue via what bond?
Thioester (High energy)
Mercaptoethylamine residue is bond to Pantothenic acid residue through what bond?
Amide bond
ATP is bond to Pantothenic acid residue via what bond?
Phosphester
What is the oxidizing agent for pyruvate dehydrogenase complex?
NAD+
Pyruvate dehydrogenase deficiency is the most common biochemical cause of congeital_____.
lactic acidosis.
Thiamine pyrophosphate (TPP) is bound to ___ which decarboxylates pyruvate.
E1
Lipoic acid is bound to___ which accepts the hydroxyethyl carbanion.
E2
Coenzyme A (CoA) is a substrate for ___ which accepts the acetyle group.
E2
Flavin adenine dinucleotide (FAD) is bound to___ which is reduced by lipoamide.
E3
Nicotinamide adenine dinucleotide (NAD+) is a substrate for___ which is reduced by FADH2.
E3
Arsenic compounds are poisonous because they sequester____?
Lipoic acid.
What is the first irreversible (RL) step?
Citrate synthase
Second irreversible step (RL) is?
Isocitrate dehydrogenase
GTP is synthesized via _____ phosphorylation.
Substrate-level
GTP can donate phophoryl group to ADP to form ATP, reversible, by which enzyme?
Nucleoside diphosphate kinase
Which enzyme of the TCA cycle is embedded in the inner mitochondrial membrane?
Succinate dehydrogenase
Where all all, beside one, enzymes located?
Mitochondrial MATRIX
What is the oxidizing agent for TCA enzymes?
NAD+
What is the product of TCA cycle?
3 NADH, 1FADH2, 1 GTP
How many ATPs are produced via 1 Acetyl CoA?
12
How many pairs of electrons are transferred during one turn?
4
TCA cycle has a ____ nature.
Amphibolic (Catabolic and anabolic pathway)
What pathways utilize TCA intermediates (Succinyl CoA mostly)?
Gluconeogenesis, GA and cholesterol synthesis, AA biosynthesis, and Porphyrin biosynthesis.
Does pyruvate carboxylase replenish TCA?
Yes!
What are the 3 anaplerotic degradative pathways?
Oxidation of odd-chain FA, Catabolism of AA isoleucine, methionine and valine. Transamination and deamination of AA.
