ETC Flashcards
ETC is present in the _____ membrane.
Inner mitochondrial membrane
The outer mitochondrial membrane is composed of what? (Highly permeable)
50% lipid and 50% protein
The inner mitochondrial membrane is highly convoluted forming the ___.
Cristae
The inner membrane is roughly __ protein.
80% protein
Cardiolipin is present in ___.
Inner mitochondrial membrane, in high concentrations.
Matrix of the mitochondrion is a gel-like solution that is __% protein.
50%
DNA in the matrix is circular or linear?
Circular
Pyruvate uses what transporter to get in mitochondria from cytosol?
Monocarboxylate
Phosphate uses what transporter to get in mitochondria from cytosol?
Dicarboxylate
Malate uses what transporter to get in mitochondria from cytosol?
Tricarboxylate
The adenine necleotide transporter and phosphate transporter are coupled, what will inhibit them?
Atractyloside (If one is inhibit, the other is also inhibited)
The requirement of oxygen makes the electron transport process the ___ chain.
Respiratory
Complex 1 is known as
NADH dehydrogenase
How many of 43 protein subunits are encoded by mitochondrial genes?
7
The iron atoms are always bount to AA___.
Cysteine
How many mitochondrial proteins are in complex 3?
0
How many mitochondrial proteins are in Cytochrome c?
1
How many mitochondrial proteins are in complex 4?
3
How many mitochondrial proteins are in complex 5?
2
Cytochromes are proteins that contain a heme group made of a ___ ring containing an atom of iron.
Protoporphyrin
The cytochromes possess a strong absorption of visible light and therefore give mitochondria their characteristic ___color.
Dark
What is another name for complex 3?
Cytochrome c reductase
What is another name for complex 4?
Cytochrome c oxidase
_____ is the only electron carrier in which the heme iron has a free ligand that can react directly with molecular oxgen.
Cytochrome a3
What is the final electron acceptor?
Oxygen
What is another name for complex 2?
Succinate dehydrogenase
What inhibits complex 1?
Amytal and rotenone
What inhibits comeplex 3?
Antimycin A
What inhibits complex 4?
CN-, CO and sodium azide
When an complex is inhibited, everything before and after is?
Reduced; oxidized
Complex 1 is the greatest ___ agent.
Reducing agent
Complex 4 is the greatest___ agent.
Oxidizing agent
How efficient is the electron transport chain?
42%
1 mol of ATP is ___kj/mol
30.5
Standard free energy of oxidation of NADH by O2 is ___kj/mol
218
What are the 3 enzymes that produce CO2?
Pyruvate dehydrogenase, isocitrate dehydrogenase, a-ketogluterate dehydrogenase
