T3: Gene expression, the lac operon and epigenetics Flashcards

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1
Q

What two types of transcription factor are there

A

Activators - promote RNA polymerase to bind to the promoter region

Repressors - prevent RNA polymerase from binding to the promoter region

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2
Q

Where does RNA polymerase bind to on DNA

A

Promoter region

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3
Q

Where do TFs bind to

A

DNA near to the promoter region

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4
Q

What are the parts of an operon

A

Regulatory gene
Promoter region
Operator region
Structural genes

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5
Q

What do regulatory genes do in operons

A

Code for a repressor transcription factor

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6
Q

What does the operator region do in an operon

A

The location where TFs bind

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7
Q

What does the promoter region do in an operon

A

Area where RNA polymerase binds to start transcription

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8
Q

How do E.coli respire

A

Aerobically using glucose but can respire lactose in a lack of glucose

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9
Q

What is the normal state of the lac operon

A

When glucose is being respired, repressor tfs bind to the operator to prevent RNA polymerase from binding so gene lac z, y and A are not expressed

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10
Q

What happens with the lac operon when the E.coli needs to use lactose for respiration

A

Lactose binds to the lac I repressor gene which changes the shape of the repressor

This allows RNA polymerase to now bind to the promoter region

Genes lac z,y and A can now be transcribed to form mRNA and produce the proteins needed to respire lactose

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11
Q

What is the link between gene combinations and plant flowering

A

The combination of genes expressed by a plant or parts of a plant determines which sturctures are formed

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12
Q

What is methylation

A

The addition of methyl groups onto CpG sites

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13
Q

What are CpG sites in DNA

A

Where a C base is next to a G base on the same strand

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14
Q

What must you call acetylation in your answer

A

HISTONE MODIFICATION

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15
Q

What does methylation do

A

Causes DNA to be more tightly wrapped around histones
Makes genes inaccessible - RNA polymerase can’t bind
So genes are not expressed

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16
Q

What does histone modification do in terms of addition of acetyl groups

A

Causes DNA to be less tightly wound around histones
So genes are accessible for RNA polymerase to bind and for the gene to be transcribed

17
Q

Name some factors that affect epigentics

A

Smoking, alcohol diet and exercise

18
Q

What are oncogenes

A

Genes that code for proteins involved in the transition between phases in the cell cycle

19
Q

What are proto-oncogenes

A

The result of mutations to oncogenes

20
Q

What is the result of proto-oncogenes

A

The cell cycle can be continually active

21
Q

What are tumour suppressor genes

A

Genes that code for suppressor proteins that stop the cell cycle if there is a problem e.g gene mutation

22
Q

What can happen if tumour suppressor genes mutate

A

Cell cycle can be continually active

23
Q

What is the BRCA1 gene responsible for

A

Codes for proteins that repair damaged DNA

24
Q

What happens if someone has 1 faulty BRCA1 allele

A

If the other becomes damaged, there will be nothing to repair damaged DNA in the body.

25
Q
A