T1 - 3.1.5.2 Semi-conservative replication Flashcards
1
Q
what is the point of DNA replication
A
to make copies to make genetically identical sister chromatids
2
Q
what joins 2 sister chromatids together
A
centromere
3
Q
what is the S phase called
A
DNA synthesis
4
Q
what is the process of semiconservative replication
A
- happens before mitosis in the S phase
- DNA is unzipped by DNA helicase, breaking weak hydrogen bonds between the bases
- Free DNA nucleotides bind through complementary base pairings such as A-T
- DNA polymerase moves in one direction forming the new strand in the 5’ –> 3’ direction and forms phosphodiester bonds between the sugar of the nucleotide and the phosphate of the next
5
Q
who developed the evidence for semiconservative replication
A
Meselson and Stahl
6
Q
how is the DNA split up
A
centrifugation
7
Q
what was the results of N-15 centrifugation
A
8
Q
what was the results of N-14 centrifugation
A
line is higher than N-15
9
Q
what was the results of N-15 and N-14 centrifuged together
A
line is in between 14 and 15
