structure of bacteria II Flashcards

1
Q

What are the differences between Prokaryotic and Eukaryotic cells?

A
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2
Q

What is the great divide between bacteria?

A

The great divide is between

Gram-positive and Gram-negative bacteria

Gram-positive bacteria are monoderm, contain one thick layer of peptidoglycan and contains teichoicand lipoteichoic acid.

Gram-negative bacteria are diderm, contains an outer membrane with lipopolysaccharides.

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3
Q

What are the subunits of the 3D peptidoglycan polymer?

A

Disaccharide N-
acetylglucosamine
N-acetylmuramic acid

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4
Q

How are the subunits of peptidoglycan bound?

A

Via cross-links between the sub-units to form long polymers that are further cross-linked by peptide cross-links

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5
Q

How is DNA contained in bacteria?

A

In nucleoids

Contains highly supercoiled DNA wrapped around proteins

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6
Q

What is the shape of nucleoids?

A

Nucleoids can change shape dramatically

Barely visible during restHighly visible when active

Can change its shape, form and function within the bacterial cytoplasm.

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7
Q

What are gas vacuoles?

A

Bacteria need to be able to contain gas intracellularly

Largest bacteria can fix nitrogen and sulphur via sulphur inclusions1mm in diameter

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8
Q

What are microcompartments?

A

Microcompartments are large, crystalline, structurally sophisticated

100-150 nm in X section

Consist of 10 000 -20 000 polypeptides of 10 to 20 types

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9
Q

What is a microcompartment shell?

A

Encasesmetabolic enzymes that catalyze reaction with toxic intermediate

Unifying structure of the microcompartments

Made of proteins having bacterial compartment domains

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10
Q

Examples of microcompartments

A

Carboxysome - metabolism

PDU compartment- metabolism of alcoholin Salmonella enterica

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11
Q

Why is the cytoskeleton important?

A

Cytoskeleton changes the shape of cells and aids the cell in cellfission

MreB (actin precursor) - elongates the cell to form rod-shaped bacteria

FtsZ - used in peptidoglycan formation

Crescentin- bends the wall to one side

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12
Q

Why can’t gram positivebacteria form vesicles?

A

Their peptidoglycan cell walls are too thick

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13
Q

How do vesicles form in gram-negative bacteria?

A
  1. Asymmetric expansion of the outer membrane relative to the inner membrane
  2. Ratio of inner to outer membrane buckles
  3. Curvature increases and the final outer membrane vesicles form
  4. Vesicles trap components in the periplasm
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14
Q

What is the periplasm?

A

Space between the outer and inner layer of the bacteria

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15
Q

What is in the most likely molecule to be found in the bacterial vesicles?

A

DNA

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16
Q

Components of the outer layer of bacteria

A
Short filaments 
S-layer proteins 
Lipoproteins 
Capsules 
Flagella
17
Q

What are bacterial capsules ?

A

Extended polysaccharide structures that protect the bacteria from immune attack

18
Q

What are filaments?

A

Adhesins

19
Q

How does the flagellum work?

A

The rotor turns via an ATP-dependent mechanism that causes the flagella to whip

20
Q

Why are flagella needed?

A

In order to swim in aqueous environment

hunt down nutrients

21
Q

Can human and bacterial flagella be distinguished?

A

Yes, via their form and function

22
Q

What are the three types of flagella?

A

Monotrichous
Peritrichous
Amphitrichous

23
Q

What are monotrichous flagells?

A

Single

24
Q

What are peritrichous flagella?

A

Multiple

25
Q

What are amphitrichous flagella?

A

One on either side of the bacteria

26
Q

What are S-layer proteins?

A

Proteins that form paracrystalline arrays on the bacterial surface

27
Q

What are the functions of S-layer proteins

A
Structural - prevent viral infection, help biofilms, protection and inhibit complement 
Receptor interaction 
Peptidoglycan remodelling 
Cell wall integrity 
Selectivity filter 
Inhibition of phagocytosis and serum killing 
Resistance to presentation 
Cell division and cell length 
Iron uptake 
Swimming 
Aggregation and bodies formation
28
Q

What are the targets of toxins in drugs attacking bacteria?

A

Alteration of cytoskeleton
Inhibition of membrane fusion
Alter membrane permeability
Interfere with cell signalling