Staining Procedures

Question 1

-commonly used dyes in bacteriology
-cationic/+ charge binds to negatively charged molecules

Answer 1

basic dyes

Question 2

-anionic dyes that binds to positively charged cells
-EOSIN, ROSE BENGAL, ACID FUSCHIN

Answer 2

acidic dyes

Question 3

staining technique:
directed towards coloring the forms and shapes

Answer 3

simple staining

Question 4

staining technique:
-divided bacteria into groups
-directed towards coloring components of elements

Answer 4

differential staining

Question 5

type of staining technique:
-GRAM STAINING
-AFB

Answer 5

differential staining

Question 6

staining technique:
directed at identification of organism

Answer 6

diagnostic antibody or DNA probe-mediated staining

Question 7

staining technique:
-demonstrate presence of diffuse capsule surrounding bacteria
-study gas vacuoles and viral morphology
-study cells sensitive to heat fixing
-appears light against dark background

Answer 7

negative staining

Question 8

type of staining technique:
INDIA INK, NIGROSIN DYE

Answer 8

negative staining

Question 9

most used differential stain in lab

Answer 9

gram stain

Question 10

theory
-Gram +: positive
-Gram -: negative

Answer 10

Mg RNA

Question 11

theory
-Gram +: less permeable to decolorizing agent
-Gram -: permeable to decolorizing agent

Answer 11

benian

Question 12

theory
-Gram +: low isoelectric point
-Gram -: high isoelectric point

Answer 12

stearn and stearn/ magnetism

Question 13

gram stain
purple/violet

Answer 13

gram +

Question 14

gram stain
pink

Answer 14

gram -

Question 15

used to stain bacteria that have high lipid content in cell walls

Answer 15

acid fast stain

Question 16

acid fast stain
red

Answer 16

acid-fast bacilli

Question 17

acid fast stain
blue-green

Answer 17

non-acid fast bacilli

Question 18

acid fast staining methods:
hot staining method

Answer 18

ziehl neelsen

Question 19

acid fast staining methods:
cold staining method

Answer 19

kinyouns

Question 20

acid fast staining methods:
differentiates M. smegmatis from M. tuberculosis

Answer 20

baumgartens

Question 21

acid fast staining methods:
selective for cell wall of AFB

Answer 21

auramine-rhodamine

Question 22

3 grams carbolfuchsin + 5% phenol

Answer 22

ziehl neelsen

Question 23

4 grams carbolfuchsin + 9% phenol

Answer 23

kinyouns

Question 24

ideal for concentrated smears and partially AFB like nocardia

Answer 24

ziehl neelsen

Question 25

mycolic acid renders resistant to decolorization

Answer 25

acid fast

Question 26

useful for identification of intestinal coccidian oocyst

Answer 26

modified kinyoun

Question 27

ideal for cryptosporidia and cyclospora parasites in stool specimens

Answer 27

modified kinyoun

Question 28

special stain:
cell wall

Answer 28

dyar stain

Question 29

special stain:
hiss,gin,anthony,welch

Answer 29

capsule

Question 30

special stain:
LAMB, neisser, albert, ljubinsky

Answer 30

metachromatic granules

Question 31

special stain:
dorner, wirtz, conklin, schaeffer-fulton

Answer 31

endospore

Question 32

special stain:
gray, leifson, fisher and conn

Answer 32

flagella

Question 33

special stain:
india ink, borris, nigrossin

Answer 33

capsule yeast cells

Question 34

special stain:
feulgen stains

Answer 34

DNA

Question 35

special stain:
fontana triboneau, levadite silver

Answer 35

spirochetes