Staining Procedures Flashcards
-commonly used dyes in bacteriology
-cationic/+ charge binds to negatively charged molecules
basic dyes
-anionic dyes that binds to positively charged cells
-EOSIN, ROSE BENGAL, ACID FUSCHIN
acidic dyes
staining technique:
directed towards coloring the forms and shapes
simple staining
staining technique:
-divided bacteria into groups
-directed towards coloring components of elements
differential staining
type of staining technique:
-GRAM STAINING
-AFB
differential staining
staining technique:
directed at identification of organism
diagnostic antibody or DNA probe-mediated staining
staining technique:
-demonstrate presence of diffuse capsule surrounding bacteria
-study gas vacuoles and viral morphology
-study cells sensitive to heat fixing
-appears light against dark background
negative staining
type of staining technique:
INDIA INK, NIGROSIN DYE
negative staining
most used differential stain in lab
gram stain
theory
-Gram +: positive
-Gram -: negative
Mg RNA
theory
-Gram +: less permeable to decolorizing agent
-Gram -: permeable to decolorizing agent
benian
theory
-Gram +: low isoelectric point
-Gram -: high isoelectric point
stearn and stearn/ magnetism
gram stain
purple/violet
gram +
gram stain
pink
gram -
used to stain bacteria that have high lipid content in cell walls
acid fast stain
acid fast stain
red
acid-fast bacilli
acid fast stain
blue-green
non-acid fast bacilli
acid fast staining methods:
hot staining method
ziehl neelsen
acid fast staining methods:
cold staining method
kinyouns
acid fast staining methods:
differentiates M. smegmatis from M. tuberculosis
baumgartens
acid fast staining methods:
selective for cell wall of AFB
auramine-rhodamine
3 grams carbolfuchsin + 5% phenol
ziehl neelsen
4 grams carbolfuchsin + 9% phenol
kinyouns
ideal for concentrated smears and partially AFB like nocardia
ziehl neelsen
mycolic acid renders resistant to decolorization
acid fast
useful for identification of intestinal coccidian oocyst
modified kinyoun
ideal for cryptosporidia and cyclospora parasites in stool specimens
modified kinyoun
special stain:
cell wall
dyar stain
special stain:
hiss,gin,anthony,welch
capsule
special stain:
LAMB, neisser, albert, ljubinsky
metachromatic granules
special stain:
dorner, wirtz, conklin, schaeffer-fulton
endospore
special stain:
gray, leifson, fisher and conn
flagella
special stain:
india ink, borris, nigrossin
capsule yeast cells
special stain:
feulgen stains
DNA
special stain:
fontana triboneau, levadite silver
spirochetes
