Culture, culture media and culture characteristics & Bacterial growth curve Flashcards
-used for appropriate growth, transport and storage of microorganisms outside the host
-utilized to observe growth patterns of microorganism
culture medium
mixture of nutrients:
carbon
hydrogen
oxygen
nitrogen
sulfur
phosphorus
buffer
(CHONSPB)
culture medium
-dyes and antimicrobials
-may be added to medium to isolate and suppress growth of other microorganism
inhibitory agents
growth of microorganism on a culture medium
culture
type of culture:
composed of only one specie
pure culture
type of culture:
more than one specie
mixed culture
type of culture:
-several species contained in a separate culture medium
-grown in volume
-academic and industrial purposes
stock culture
culture medium according to consistency:
contain 0% agar
liquid medium
culture medium according to consistency:
0.5-1% agar
eg: SIM
semi-solid medium
culture medium according to consistency:
2-3% agar
eg: TSI, CAP
solid medium
sulfated polymer derived from red algae
agar
culture medium according to composition:
medium where all components are KNOWN
synthetic/defined medium
culture medium according to composition:
composed of some UNKNOWN substances, for isolation
non-synthetic/complex medium
culture medium according to composition:
used for obligate intracellular bacteria
tissue culture medium
culture medium according to use:
-routinely used without added supplement
-growth of NONFASTIDIOUS bacteria
simple/general purpose/supportive media
culture medium according to use:
Nutrient agar (NA)
tyrpticase soy broth (TSB)
simple/general purpose/supportive media
culture medium according to use:
-contains specific nutrients
-LIQUID-type media
enrichment broth
culture medium according to use:
-added supplements necessary for growth of FASTIDIOUS organism
-SOLID-type media
enriched media/non selective media
culture medium according to use:
BAP
CAP
enriched media/non selective media
standard nonselective culture medium
BAP
with horse blood used for isolation of Haemophilus
CAP
allows visualization of metabolic differences between groups of bacteria
differential media
differentiates lactose fermenters (PINK) from non lactose fermenters (COLORLESS)
mac conkey
differentiate hemolytic pattern (streptococci)
BAP
media incorporated with antibiotics, dyes, chemical to inhibit the growth of other organisms
selective media
selective media:
gentamicin blood agar
streptococci
selective media:
bacitracin chocolate agar
haemophilus
selective media:
blood agar plate with ampicillin
aeromonas
selective media:
phenyl ethyl alcohol
gram + cocci
selective media:
columbia agar with CNA
gram + bacteria
selective media:
gram negative broth
salmonella, shigella
selective media:
thayer martin agar
neisseria gonorrheae
inhibitory substance:
alcohol and chloral hydrate
swarming bacteria
inhibitory substance:
potassium tellurite and sodium azide
gram - bacteria
inhibitory substance:
crystal/gentian violet, basic/carbol fuschin and bile salt
gram + bacteria
used to isolate bacteria with specific growth requirements
special media
agar that contains bile salt and dyes
for non enteric pathogens that inhibits normal flora of lower GIT
HE agar
agar that contains 0.25% sodium deoxycholate salt and sodium thiosulfate
to differentiate salmonella (red WITH BLACK centers)
from shigella (red WITHOUT BLACK centers)
XLD agar
components of anaerobic chamber:
used as filler
nitrogen gas
components of anaerobic chamber:
removes residual oxygen
palladium pellets
components of anaerobic chamber:
absorbs the water
silica gel/dessicant
components of anaerobic chamber:
becomes colorless in absence of oxygen
methylene blue/ reazurin
the ideal anaerobic incubation system is -
anaerobic chamber
production of H2S from anaerobic results to a
poisoned catalyst
inoculation technique:
most commonly performed
streaking
inoculation technique:
-technique applied to semi-solid medium like SIM
-inoculate streptococci into BAP
stabbing
inoculation technique:
used to inoculate organism into slant and butt tube medium TSI
stab and streak
inoculation technique:
allows medium to solidify
pour plate
doubling of cell number
generation
time required for a bacteria to double its population
generation time/doubling time
bacterial growth curve:
-period with no cell division, no abrupt increase
-start of biosynthesis
-adjusting to new environment
lag phase/period of rejuvenescence
bacterial growth curve:
-period where microorganisms ae actively growing and dividing
-utilized in physiological and biochemical testing
log /exponential phase (balance growth)
bacterial growth curve:
-period where there is cessation of bacterial growth
death/decline phase
no cell division occurs in the presence of a nuclear division
coenocytic
method for measuring bacterial growth:
does not distinguish between live and dead cells
microscopic count
method for measuring bacterial growth:
-most commonly used method
-measures number of viable cells
-can determine CFU/mL of bacteria
plate count
method for measuring bacterial growth:
-requires 10-100M
-used to prepare standard inoculums for antimicrobial testing
turbidimetric method
method for measuring bacterial growth:
for filamentous organisms (fungi)
dry weight determination
