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BACTERIOLOGY AND AUBF > Culture, culture media and culture characteristics & Bacterial growth curve > Flashcards

Culture, culture media and culture characteristics & Bacterial growth curve Flashcards

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1
Q

-used for appropriate growth, transport and storage of microorganisms outside the host
-utilized to observe growth patterns of microorganism

A

culture medium

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2
Q

mixture of nutrients:
carbon
hydrogen
oxygen
nitrogen
sulfur
phosphorus
buffer
(CHONSPB)

A

culture medium

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3
Q

-dyes and antimicrobials
-may be added to medium to isolate and suppress growth of other microorganism

A

inhibitory agents

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4
Q

growth of microorganism on a culture medium

A

culture

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5
Q

type of culture:
composed of only one specie

A

pure culture

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6
Q

type of culture:
more than one specie

A

mixed culture

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7
Q

type of culture:
-several species contained in a separate culture medium
-grown in volume
-academic and industrial purposes

A

stock culture

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8
Q

culture medium according to consistency:
contain 0% agar

A

liquid medium

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9
Q

culture medium according to consistency:
0.5-1% agar
eg: SIM

A

semi-solid medium

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10
Q

culture medium according to consistency:
2-3% agar
eg: TSI, CAP

A

solid medium

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11
Q

sulfated polymer derived from red algae

A

agar

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12
Q

culture medium according to composition:
medium where all components are KNOWN

A

synthetic/defined medium

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13
Q

culture medium according to composition:
composed of some UNKNOWN substances, for isolation

A

non-synthetic/complex medium

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14
Q

culture medium according to composition:
used for obligate intracellular bacteria

A

tissue culture medium

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15
Q

culture medium according to use:
-routinely used without added supplement
-growth of NONFASTIDIOUS bacteria

A

simple/general purpose/supportive media

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16
Q

culture medium according to use:
Nutrient agar (NA)
tyrpticase soy broth (TSB)

A

simple/general purpose/supportive media

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17
Q

culture medium according to use:
-contains specific nutrients
-LIQUID-type media

A

enrichment broth

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18
Q

culture medium according to use:
-added supplements necessary for growth of FASTIDIOUS organism
-SOLID-type media

A

enriched media/non selective media

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19
Q

culture medium according to use:
BAP
CAP

A

enriched media/non selective media

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20
Q

standard nonselective culture medium

A

BAP

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21
Q

with horse blood used for isolation of Haemophilus

A

CAP

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22
Q

allows visualization of metabolic differences between groups of bacteria

A

differential media

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23
Q

differentiates lactose fermenters (PINK) from non lactose fermenters (COLORLESS)

A

mac conkey

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24
Q

differentiate hemolytic pattern (streptococci)

25
media incorporated with antibiotics, dyes, chemical to inhibit the growth of other organisms
selective media
26
selective media: gentamicin blood agar
streptococci
27
selective media: bacitracin chocolate agar
haemophilus
28
selective media: blood agar plate with ampicillin
aeromonas
29
selective media: phenyl ethyl alcohol
gram + cocci
30
selective media: columbia agar with CNA
gram + bacteria
31
selective media: gram negative broth
salmonella, shigella
32
selective media: thayer martin agar
neisseria gonorrheae
33
inhibitory substance: alcohol and chloral hydrate
swarming bacteria
34
inhibitory substance: potassium tellurite and sodium azide
gram - bacteria
35
inhibitory substance: crystal/gentian violet, basic/carbol fuschin and bile salt
gram + bacteria
36
used to isolate bacteria with specific growth requirements
special media
37
agar that contains bile salt and dyes for non enteric pathogens that inhibits normal flora of lower GIT
HE agar
38
agar that contains 0.25% sodium deoxycholate salt and sodium thiosulfate to differentiate salmonella (red WITH BLACK centers) from shigella (red WITHOUT BLACK centers)
XLD agar
39
components of anaerobic chamber: used as filler
nitrogen gas
40
components of anaerobic chamber: removes residual oxygen
palladium pellets
41
components of anaerobic chamber: absorbs the water
silica gel/dessicant
42
components of anaerobic chamber: becomes colorless in absence of oxygen
methylene blue/ reazurin
43
the ideal anaerobic incubation system is -
anaerobic chamber
44
production of H2S from anaerobic results to a
poisoned catalyst
45
inoculation technique: most commonly performed
streaking
46
inoculation technique: -technique applied to semi-solid medium like SIM -inoculate streptococci into BAP
stabbing
47
inoculation technique: used to inoculate organism into slant and butt tube medium TSI
stab and streak
48
inoculation technique: allows medium to solidify
pour plate
49
doubling of cell number
generation
50
time required for a bacteria to double its population
generation time/doubling time
51
bacterial growth curve: -period with no cell division, no abrupt increase -start of biosynthesis -adjusting to new environment
lag phase/period of rejuvenescence
52
bacterial growth curve: -period where microorganisms ae actively growing and dividing -utilized in physiological and biochemical testing
log /exponential phase (balance growth)
53
bacterial growth curve: -period where there is cessation of bacterial growth
death/decline phase
54
no cell division occurs in the presence of a nuclear division
coenocytic
55
method for measuring bacterial growth: does not distinguish between live and dead cells
microscopic count
56
method for measuring bacterial growth: -most commonly used method -measures number of viable cells -can determine CFU/mL of bacteria
plate count
57
method for measuring bacterial growth: -requires 10-100M -used to prepare standard inoculums for antimicrobial testing
turbidimetric method
58
method for measuring bacterial growth: for filamentous organisms (fungi)
dry weight determination

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