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BACTERIOLOGY AND AUBF > Culture, culture media and culture characteristics & Bacterial growth curve > Flashcards

Culture, culture media and culture characteristics & Bacterial growth curve Flashcards

1
Q

-used for appropriate growth, transport and storage of microorganisms outside the host
-utilized to observe growth patterns of microorganism

A

culture medium

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2
Q

mixture of nutrients:
carbon
hydrogen
oxygen
nitrogen
sulfur
phosphorus
buffer
(CHONSPB)

A

culture medium

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3
Q

-dyes and antimicrobials
-may be added to medium to isolate and suppress growth of other microorganism

A

inhibitory agents

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4
Q

growth of microorganism on a culture medium

A

culture

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5
Q

type of culture:
composed of only one specie

A

pure culture

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6
Q

type of culture:
more than one specie

A

mixed culture

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7
Q

type of culture:
-several species contained in a separate culture medium
-grown in volume
-academic and industrial purposes

A

stock culture

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8
Q

culture medium according to consistency:
contain 0% agar

A

liquid medium

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9
Q

culture medium according to consistency:
0.5-1% agar
eg: SIM

A

semi-solid medium

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10
Q

culture medium according to consistency:
2-3% agar
eg: TSI, CAP

A

solid medium

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11
Q

sulfated polymer derived from red algae

A

agar

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12
Q

culture medium according to composition:
medium where all components are KNOWN

A

synthetic/defined medium

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13
Q

culture medium according to composition:
composed of some UNKNOWN substances, for isolation

A

non-synthetic/complex medium

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14
Q

culture medium according to composition:
used for obligate intracellular bacteria

A

tissue culture medium

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15
Q

culture medium according to use:
-routinely used without added supplement
-growth of NONFASTIDIOUS bacteria

A

simple/general purpose/supportive media

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16
Q

culture medium according to use:
Nutrient agar (NA)
tyrpticase soy broth (TSB)

A

simple/general purpose/supportive media

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17
Q

culture medium according to use:
-contains specific nutrients
-LIQUID-type media

A

enrichment broth

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18
Q

culture medium according to use:
-added supplements necessary for growth of FASTIDIOUS organism
-SOLID-type media

A

enriched media/non selective media

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19
Q

culture medium according to use:
BAP
CAP

A

enriched media/non selective media

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20
Q

standard nonselective culture medium

A

BAP

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21
Q

with horse blood used for isolation of Haemophilus

A

CAP

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22
Q

allows visualization of metabolic differences between groups of bacteria

A

differential media

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23
Q

differentiates lactose fermenters (PINK) from non lactose fermenters (COLORLESS)

A

mac conkey

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24
Q

differentiate hemolytic pattern (streptococci)

A

BAP

25
Q

media incorporated with antibiotics, dyes, chemical to inhibit the growth of other organisms

A

selective media

26
Q

selective media:
gentamicin blood agar

A

streptococci

27
Q

selective media:
bacitracin chocolate agar

A

haemophilus

28
Q

selective media:
blood agar plate with ampicillin

A

aeromonas

29
Q

selective media:
phenyl ethyl alcohol

A

gram + cocci

30
Q

selective media:
columbia agar with CNA

A

gram + bacteria

31
Q

selective media:
gram negative broth

A

salmonella, shigella

32
Q

selective media:
thayer martin agar

A

neisseria gonorrheae

33
Q

inhibitory substance:
alcohol and chloral hydrate

A

swarming bacteria

34
Q

inhibitory substance:
potassium tellurite and sodium azide

A

gram - bacteria

35
Q

inhibitory substance:
crystal/gentian violet, basic/carbol fuschin and bile salt

A

gram + bacteria

36
Q

used to isolate bacteria with specific growth requirements

A

special media

37
Q

agar that contains bile salt and dyes
for non enteric pathogens that inhibits normal flora of lower GIT

A

HE agar

38
Q

agar that contains 0.25% sodium deoxycholate salt and sodium thiosulfate
to differentiate salmonella (red WITH BLACK centers)
from shigella (red WITHOUT BLACK centers)

A

XLD agar

39
Q

components of anaerobic chamber:
used as filler

A

nitrogen gas

40
Q

components of anaerobic chamber:
removes residual oxygen

A

palladium pellets

41
Q

components of anaerobic chamber:
absorbs the water

A

silica gel/dessicant

42
Q

components of anaerobic chamber:
becomes colorless in absence of oxygen

A

methylene blue/ reazurin

43
Q

the ideal anaerobic incubation system is -

A

anaerobic chamber

44
Q

production of H2S from anaerobic results to a

A

poisoned catalyst

45
Q

inoculation technique:
most commonly performed

A

streaking

46
Q

inoculation technique:
-technique applied to semi-solid medium like SIM
-inoculate streptococci into BAP

A

stabbing

47
Q

inoculation technique:
used to inoculate organism into slant and butt tube medium TSI

A

stab and streak

48
Q

inoculation technique:
allows medium to solidify

A

pour plate

49
Q

doubling of cell number

A

generation

50
Q

time required for a bacteria to double its population

A

generation time/doubling time

51
Q

bacterial growth curve:
-period with no cell division, no abrupt increase
-start of biosynthesis
-adjusting to new environment

A

lag phase/period of rejuvenescence

52
Q

bacterial growth curve:
-period where microorganisms ae actively growing and dividing
-utilized in physiological and biochemical testing

A

log /exponential phase (balance growth)

53
Q

bacterial growth curve:
-period where there is cessation of bacterial growth

A

death/decline phase

54
Q

no cell division occurs in the presence of a nuclear division

A

coenocytic

55
Q

method for measuring bacterial growth:
does not distinguish between live and dead cells

A

microscopic count

56
Q

method for measuring bacterial growth:
-most commonly used method
-measures number of viable cells
-can determine CFU/mL of bacteria

A

plate count

57
Q

method for measuring bacterial growth:
-requires 10-100M
-used to prepare standard inoculums for antimicrobial testing

A

turbidimetric method

58
Q

method for measuring bacterial growth:
for filamentous organisms (fungi)

A

dry weight determination

BACTERIOLOGY AND AUBF (12 decks)

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