Microbial nutrition, growth requirements and bacterial metabolism Flashcards

1
Q

-requires oxygen and grows well in room air
-15-21% oxygen, 1% CO2

A

aerobe

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2
Q

eg:
pseudomonas
bordetella
brucella
mycobacteria
PBBM

A

obligate aerobic

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3
Q

-does not require presence of oxygen
-dies in presence of oxygen

A

obligate anaerobic

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4
Q

eg:
clostridium
bacteroides
(CBac)

A

obligate anaerobic

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5
Q

-most clinically significant organism
-grows in either presence or absence of oxygen
-does not require oxygen but grows well with oxygen

A

facultative anaerobe

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6
Q

eg: enterobacteria

A

facultative anaerobe

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7
Q

obligate aerobes and facultative anaerobes contain protective enzymes (2) against toxic effect of oxygen

A

superoxidase dismutase
catalase

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8
Q

-can survive in presence of oxygen but will not perform metabolic process unless placed in anerobic environment

A

aerotolerant anaerobe

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9
Q

eg:
propionibacterium
lactobaciullus
(ProLac)

A

aerotolerant anaerobe

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10
Q

organism that requires 2-10% oxygen for growth

A

microaerophile

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11
Q

eg:
campylobacter
treponema pallidum
(CT)

A

microaerophile

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12
Q

requires 5-10% CO2

A

capnophiles

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13
Q

eg.
haemophilus influenzae
neisseria gonorrheae
streptococcus pneumoniae
(HaNeS)

A

capnophiles

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14
Q

CO2 % needed by aerobic bacteria

A

0.03%

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15
Q

according to carbon source:
use CO2 as sole source of carbon

A

autotrophs

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16
Q

according to carbon source:
use reduced, preformed organic molecules from other bacteria

A

heterotrophs

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17
Q

according to energy source:
organisms that use light

A

phototrophs

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18
Q

according to energy source:
organisms that use the energy produced by the oxidation of organic or inorganic compounds

A

chemotrophs

19
Q

according to electron source:
-they reduce inorganic molecules
-same as autotrophs

A

litotrophs

20
Q

according to electron source:
-require organic substances for growth and multiplication
-ALL BACTERIA THAT INHABIT HUMAN BODY
-same as heterotrophs

A

organotrophs

21
Q

obtain energy either by photosyntetically or oxidatively

A

autotrophs
litotrophs

22
Q

obtain energy by oxidation or fermenting organic substances such as glucose

A

heterotrophs
organotrophs

23
Q

requires additional substances such as vitamins, purines, pyrimidines and hemoglobin for growth and survival

A

fastidious bacteria

24
Q

require dead organic substances

A

saprophytes

25
Q

requires organic substance from living tissues

A

parasites

26
Q

optimum temperature for most bacteria

A

35-37c

27
Q

according to temperature:
grows well at 0c to a maximum of 20c

A

psychrophile/cryophile

28
Q

eg:
listeria monocytogenes
yersinia enterocolitica

A

psychrophile/cryophile

29
Q

according to temperature:
grows between 20-45c
commonly encountered pathogenic bacteria in lab

A

mesophile

30
Q

according to temperature:
grows beterrn 50-125c

A

thermophiles/hyperthemophile

31
Q

according to temperature:
prokaryotes that are able to live at unusual conditions
eg: bacillus infernus

A

extremophiles

32
Q

term:
the lowest minimum time required to kill organism in a constant temperature

A

thermal death time

33
Q

term:
the lowest temperature required to kill organism at a constant time

A

thermal death point

34
Q

measure of the hydrogen ion concentration of an organism’s environment

A

pH requirement

35
Q

pH requirement:
pH 0-5.5

A

acidophile

36
Q

pH requirement:
pH 5.5-8.0

A

neutrophile

37
Q

pH requirement:
pH 8.5-11.5

A

alkalophile

38
Q

pH requirement:
most clinically significant bacteria

A

neutrophile

39
Q

optimum pH for most pathogenic bacteria is between -

A

6.5-7.5

40
Q

requirement important for bacterial growth

A

moisture

41
Q

requires increased concentration of sodium chloride

A

halophiles

42
Q

pressure requirement:
organism that grow rapidly in presence of high pressure

A

barophilic

43
Q

substances required by fastidious bacteria for their growth and multiplication

A

growth factors:
amino acids
vitamins
purines
pyrimidines