Microscopy

Question 1

first person to observe and describe microorganism ‘animalcules’

Answer 1

anton van leeuwenhoeok

Question 2

great founders of microbiology

Answer 2

louis pasteur
robert koch

Question 3

image should remain in focus when objectives are changed

Answer 3

parfocal

Question 4

microscope:
-most commonly used
-dark image, bright background

Answer 4

bright field microscopy

Question 5

magnification of ocular lens

objective lenses:
LPF- 10x, 100x
HPF-40x, 400x
OIO-100, 1000x

Answer 5

10x

Question 6

has same refractive index as glass, becomes part of optics. it enhances resolution fill the spcae between objective lens and glass slide

Answer 6

cedar wood oil

Question 7

part of microscope:
compensate for observer’s eyesight in left and right eye

Answer 7

diopter adjustment ring

Question 8

part of microscope:
determines distance between eyes

Answer 8

interpuppilary distance scale

Question 9

part of microscope:
focuses light through specimen

Answer 9

condenser

Question 10

-used to identify fungi in growth cultures
-detailed examination of internal structures

Answer 10

phase contrast microscopy

Question 11

-blocks light, directs light to hit specimen
-detect spirochetes (treponema pallidum)
-examine UNSATINED microorganisms suspended in liquid

Answer 11

dark field microscopy

Question 12

objects maller than 0.2um, up to 100,000x magnification
-useful for studying viruses

Answer 12

electron microscopy

Question 13

relative velocity at which light passes through

Answer 13

refractive index

Question 14

ability of lenses to separate closely distant ibjects

Answer 14

resolving power

Question 15

state to differentiate true motility from brownian
-wet mount preparation
-hanging drop preparation

Answer 15

living state

Question 16

bacteria in a definite direction

Answer 16

true motility

Question 17

bacteria back and fourth

Answer 17

brownian movement

Question 18

state for staining

Answer 18

fixed state