Specimen Procurement, Processing & Data Collection Flashcards
Order of Draw
- Aerobic
- Anaerobic
- Yellow
- Light Blue
- Red
- Orange
- Gold
- Light Green
- Lime
- Green
- Lavender
- Pink
- Royal Blue
- Tan
- White
- Grey
- Black
Additives: Aerobic
Culture
Additives: Anaerobic
Culture
Additives/Inversions: Yellow (Sterile)
SPS (Sodium Polyanethol Sulfonate
8x
Additives/Inversions: Yellow (Non-Sterile)
Acid Citrate Dextrose
8x
Additives/Inversions: Light Blue
Sodium Citrate:
- Discard tube first to get rid of air bubbles
- DO NOT UNDERFILL
3-4x
Additives/Inversions: Red
None
0x
Clot Activator
5x
Additives/Inversions: Orange
Thrombin
8x
Additives/Inversions: Gold
SST (Serum Separating Tube):
- Thixotropic gel
5x
Additives/Inversions: Light Green
PST (Plasma Separating Tube):
- Lithium Heparin
8x
Additives/Inversions: Lime
Barricore
Additives/Inversions: Dark Green
Sodium Heparin
8x
Additives/Inversions: Lavender
EDTA
8x
Additives/Inversions: Pink
EDTA K2:
- Spray-coated
8x
Additives/Inversions: Royal Blue
EDTA
Additives/Inversions: Tan
EDTA
8x
Additives/Inversions: White
EDTA
Additives/Inversions: Grey
Sodium Fluoride
8x
Additives/Inversions: Black
Sodium Citrate
10x
What is Plasma?
Unclotted liquid component of blood
What is Serum?
Clotted liquid component of blood
Collection Type: Arm
Antecubital Fossa:
- Median cubital (middle)
- Cephalic (outer side of arm)
- Brachial (inner side of arm)
Collection Type: Hand
Butterfly/winged infusion
Collection Type: Finger
Microtainer or Lancet
(wipe away first drop)
Collection Type: Heel
- Prewarm
- Never more than 2mm depth
- Lateral heel
Needle Types: Green
21g - Used mainly for patients on blood thinners
Needle Types: Black
22g - Standard
Needle Types: Blue
23g
Lancet Types: Blue
Adult
Lancet Types: Pink
Neonate or Geriatric
Special Considerations: Edema
Pressure to move fluid away from tourniquet/puncture site or select other side
Special Considerations: Hematoma/Bruising
Select other side
Special Considerations: IV Placement
- Opposite arm
or - IV off for 5 mins
- Select site distal to IV
- Select different vein
Special Considerations: Masectomy
Opposite side of the body
Special Considerations: Petechiae
Red spots form, from too tight/too long tourniquet
Special Considerations: Blood Alcohol Testing
Use chlorhexidine, NOT alcohol based cleansers
Special Considerations: Hemolysis
Affects:
- K (potassium)
- LD (lactate dehydrogenase)
- AST (liver function)
- Mg (magnesium)
- Iron
- B12
- Folate
First Aid: Fainting
- Remove tourniquet and needle
- Cap needle asap and safely
- Tend to patient
First Aid: Hematoma/Extended Bleeding
Pressure for 5 minutes then check
First Aid: Tourniquet
No longer than 1 min
First Aid: CAB
C - Compressions
A - Airway
B - Breathing
Specimen Storage: Light Sensitive
- Bilirubin
- Erythrocyte Protoporphyrin
- Carotene
- Vitamin A
Specimen Storage: Warm
- Cold Agglutinin
- Cryoglobulin
Specimen Storage: Chilled
- ACTH (adrenocorticotropic hormone)
- Acetone
- ACE (angiotensin converting enzyme)
- Angio
- Aldosterone
- Ammonia
- Catecholamine
- Free Fatty Acids
- Lactate
- Pyruvate
- Renin Activity
- DHEA (dehydroepiandrosterone)
- Vitamin C
Chain of Custody
- Record every person that touches specimen
- Ensure seal/paperwork
Precautions: Contact
Infection spread by touch
Precautions: Droplet
Infection spread by cough/sneeze particles
Precautions: Airborne
Infection spread in the air
Precautions: Nosocomical
Hospital acquired infection (HAI)
Additives: EDTA
- Maintains cell shape
- Prevents clotting by binding with calcium
Additives: Heparin
Inactivates thrombin and factor Xa
Additives: Sodium Citrate
- Mild reaction binding with calcium
- Preserves factor V
Additives: Sodium Fluoride
Preserves glucose
Storage: Fungal Cultures
Stored at RT (room temp)
Storage: Blood/CSF
Can be stored at 35C (body temp)
Storage: Urine
Stored at 4C
Assorted Acronyms: GTT
Glucose tolerance test
Assorted Acronyms: OGTT
Oral glucose tolerance test
Assorted Acronyms: LH
Luteinizing Hormone
Assorted Acronyms: PSA
Prostate Specific Antigen
Assorted Acronyms: QNS
Quantity not Sufficient
Assorted Acronyms: ETS
Evacuated Tube System
Assorted Acronyms: BT
Bleeding Time
Assorted Acronyms: CBC
Complete Blood Count
Assorted Acronyms: ACD
Acid Citrate Dextrose
Assorted Acronyms: CK
Creatine Kinase
Assorted Acronyms: TSH
Thyroid Stimulating Hormone
Assorted Acronyms: PP
Post Prandial
Assorted Acronyms: PLT
Platelets
Assorted Acronyms: XDP
Serum Crosslinked Fibrin
Assorted Acronyms: HCT
Hematocrit
Assorted Acronyms: BBP
Blood Borne Pathogen
Assorted Acronyms: ESR
Erythrocyte Sedimentation Rate