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Blood > Smears and Staining > Flashcards

Smears and Staining Flashcards

1
Q

Coverslip Technique

A
  • Even distribution, especially WBCs
  • Doesn’t rupture cells as easily
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2
Q

Stain #1

A
  • FIX
  • Methyl Alcohol
  • Solidifies Cells
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3
Q

Stain #2

A
  • Eosin (red)
  • Stains the basic parts of the cell (cytoplasm)
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4
Q

Stain #3

A

Stains the nucleic acid

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5
Q

Erythrocytic Indices

A

Help classify anemias
* MCV
* MCH
* MCHC

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6
Q

Erythrocytic Indices

MCV

A

Mean Corpuscular Volume
* size

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7
Q

MCV Calculations

A
  • PCV x 10 divided by RBC
  • Label in fL (femtoliters)
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8
Q

MCV Normal Range

A
  • Dogs: 60-80 fL
  • Cats: 40-60 fL
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9
Q

Erythrocytic Indices

MCH

A

Mean Corpuscular Hemoglobin
* weight of hemoglobin

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10
Q

MCH Calculations

and Normal Range

A
  • Hgb x 10 divided by RBC
  • Normal = 20 pg (picograms)
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11
Q

Erythrocytic Indices

MCHC

A
  • Mean Hemoglobin Concentration
  • Average amount of hemoglobin in volume of blood
  • Description of color of RBC
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12
Q

MCHC Calculations

A
  • Hbg x 100 divided PCV
  • Normal = 30-36 g/dL or %
  • Most accurate
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13
Q

Normocytic

A
  • Part of MCV
  • Normal sized RBC population
  • Determines health and nonregenerative anemia
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14
Q

Macrocytic

A
  • Part of MCV (elevated)
  • Increased size of RBC
  • Seen in regenerative anemia, Malamutes, and Mini Poodles, and FeLV
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15
Q

Microcytic

A
  • Part of MCV (decreased)
  • Decreased size of RBC
  • Seen with iron deficiency
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16
Q

Normochromic

A
  • Part of MCHC
  • Normal color of RBCs
  • Determines health and nonregenerative anemia
17
Q

Hypochromic

A
  • Part of MCHC (decreased)
  • Average RBCs are paler than normal
  • Increased central pallor
  • Decreased stain intensity - decreased hemoglobin
  • Seen with marked regenerative anemia and severe iron deficiency
  • Almost always seen with microcytosis
18
Q

Erythrocytes

A
  • Red Blood Cells
  • Bind with hemoglobin
  • Formation in bone marrow
  • Circulation of 90-120 days
19
Q

Anemia Causes

A
  1. Blood loss
  2. Hemolysis
  3. Lack of production
20
Q

Anemia

General

A

Most common RBC distribution in animals

21
Q

Regenerative Anemia

Characteristics (5)

A
  • Decreased PCV
  • Increased reticulocyte count
  • Polychromasia and Anisocytosis in blood film
  • Elevated MCV
  • Decreased MCHC
22
Q

Non-Regenerative Anemia

Characteristics

A
  • Decreased PCV
  • No reticulocytosis
  • Needs a bone marrow exam

Blood (11 decks)

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