Slide prep & microscopy basics

Question 1

steps for tissue preparation

Answer 1

fixation, embedding & sectioning, mounting, staining

Question 2

purpose of fixation

Answer 2

preserve tissue

Question 3

purpose of embedding & sectioning

Answer 3

allows cutting of thin slides, lipids are lost

Question 4

Purpose of Mounting

Answer 4

attaching slide via heat or adhesive

Question 5

Eosin Dye

Answer 5

stains non-mucin protein & collagen red

Question 6

Hematoxylin

Answer 6

stains DNA, RNA, and ER blue

Question 7

Feulgen

Answer 7

stains DNA, used to distinguish between DNA & RNA

Question 8

Periodic acid-Schiff (PAS)

Answer 8

stains basement membrane & glycogen red

Question 9

Alcian blue

Answer 9

stains mucin blue

Question 10

Azan

Answer 10

stains nuclei, RBC’s and muscle red

stains mucin blue

Question 11

Congo red

Answer 11

stains amyloid red

Question 12

Giemsa

Answer 12

stains RBC’s pink

stains nuclei blue

Question 13

Masson’s Trichrome

Answer 13

stains nuclei blue

stains connective tissue blue/green

stains keratin, muscle, and hyaline red

Question 14

Orange G

Answer 14

stains RBC’s orange

Question 15

Sudan black & osmium

Answer 15

stains myelin brownish-black

Question 16

Marilyn Monroe mnemonic

Answer 16

Marilyn- macular dystrophy
Monroe-Mucopolysaccharide
Always- Alcian Blue stain
Gets- Granular Dystrophy
Her- Hyaline
Man in- Masson Trichrome
Los- Lattice Dystrophy
Angeles- Amyloid
California- Congo red

Question 17

Direct binding

Answer 17

tagged antibody directly binds antigen

Question 18

Indirect binding

Answer 18

non-tagged antibody binds antigen, tagged antibodies bind untagged antibody

Question 19

Hybridization

Answer 19

detected via radioactive labels or fluorescein

Question 20

autoradiography

Answer 20

radioactive labeling of precursors

Question 21

types of microscopes (least to most magnified)

Answer 21

human eye, bright-field, scanning electron, transmission electron, atomic force