Skin Puncture

Question 1

A technique used to obtain blood specimen when venipuncture or other collection are not appropriate.

Answer 1

Skin Puncture

Question 2

Blood sample collected is a mixture of:

Answer 2

Capillary Blood
Arterial Blood
Venous Blood

Question 3

Sites of Skin Puncture: (Be Specific)

Answer 3

Finger (middle or ring finger)
Earlobe
If the patient is less than 1 year old: Lateral portion of the plantar surface of the foot

Question 4

Sites to avoid:

Answer 4

❖Inflamed and pallor areas
❖Cold and cyanotic areas
❖Congested and edematous
❖Scarred and heavily calloused areas

Question 5

Advantages of Skin Puncture using the Finger:

Answer 5

❖Easily accessible to the operator
❖Easily to manipulate
❖Ideal for peripheral blood smears

Question 6

Disadvantages of Skin Puncture:

Answer 6

❖Less amount of blood can be obtained
❖Additional and repeated test cannot be done
❖Blood obtained by skin puncture hemolyzes easily

Question 7

When doing a Skin Puncture, the depth of the lancets must be _____ in length.

Answer 7

2-3mm

Question 8

Lancets used for heel puncture in full term newborn must not exceeds _____ in length.

Answer 8

2-4mm

Question 9

Write down the step-by-step procedure in Skin Puncture:

Answer 9

1. Select the puncture site and warm the puncture site.
2. Cleanse the puncture site with 70% alcohol using concentric circles.
3. Grasp the finger and make a quick prick with the use of sterile lancet.
4. Using sterile dry gauze, wipe away the first drop of blood.
5. Obtain drops of blood for the desired examination.
6. Place cotton pad on the puncture site.
7. Instruct the patient to elevate the puncture site and apply pressure until bleeding has stopped.

Question 10

When cleansing the puncture site, what do we use and in what motion is done?

Answer 10

70% alcohol in concentric circles

Question 11

In the Skin Puncture, do we need to wipe away the first drop of blood or not?

Answer 11

We need to wipe away the first drop of blood

Question 12

Order of Draw for Skin Puncture:

Answer 12

1. Tube for blood gas analysis
2. Slides, unless made from specimen in the EDTA micro collection tube
3. EDTA micro collection tube
4. Other micro collection tubes with anticoagulants
5. Serum micro collection tubes

Question 13

Who recommended this following Order of Draw:

1. Tube for blood gas analysis
2. Slides, unless made from a specimen in the EDTA
   microcollection tube
3. EDTA microcollection tube
4. Other microcollection tubes with anticoagulants
5. Serum microcollection tubes

Answer 13

Clinical and Laboratory Standards Institute

Question 14

CLSI recommendation order of Draw:

Answer 14

1. Tube for blood gas analysis
2. Slides, unless made from a specimen in the EDTA microcollection tube
3. EDTA microcollection tube
4. Other microcollection tubes with anticoagulants
5. Serum microcollection tubes

Question 15

Identify the Step Number:

Select the puncture site.

Answer 15

Step 1

Question 16

Identify the Step Number:

Warm the puncture site.

Answer 16

Step 2

Question 17

Identify the Step Number:

Cleanse the puncture site with 70% alcohol using concentric circles.

Answer 17

Step 3

Question 18

Identify the Step Number:

Grasp the finger and make a quick prick with the use of sterile lancet.

Answer 18

Step 4

Question 19

Identify the Step Number:

Using sterile dry gauze, wipe away the first drop of blood.

Answer 19

Step 5

Question 20

Identify the Step Number:

Obtain drops of blood for the desired examination.

Answer 20

Step 6

Question 21

Identify the Step Number:

Place cotton pad on the puncture.

Answer 21

Step 7

Question 22

Identify the Step Number:

Instruct the patient to elevate the puncture site and apply pressure until bleeding has stopped.

Answer 22

Step 8