Acid Hematin Flashcards
It is the red iron-bearing protein contained within the erythrocytes in normal blood.
Hemoglobin
What is the function of Hemoglobin?
It is the carrier of oxygen from the lungs to the tissue where it readily releases this oxygen to the tissues and then returns to the lungs to combine with more oxygen.
Complete the statement:
_____ molecules are conjugated with 2 pairs of globin chains.
4 heme
Complete the statement:
Four heme molecules are conjugated with _____ of globin chains.
2 pairs
Complete the statement:
_____ represents the colored component
Heme
Complete the statement:
_____ is a complex protein.
Globin
Complete the statement:
The molecular weight of one Hb molecule is _____
64,458 daltons
Hemoglobin results are affected by the following
factors:
Age
Sex
Altitude of the locality
Methods for determining Hemoglobin in Blood:
Copper Sulfate or Specific Gravity Method
Gasometric Method or Oxygen Capacity Method
Chemical Method or Iron Content Method
Colorimetric Method
Best method for measuring plasma hemoglobin.
Oxyhemoglobin Method
The best method considered by hematologist.
Cyanmethemoglobin or HiCN Method
The Hemoglobin Determination Test is used to:
- Screen for disease associated with anemia
- Determine the severity of anemia
- Follow the response to treatment for anemia
- Evaluate polycythemia
Increased Hemoglobin is also called as _____
Hyperchromia
Decresed Hemoglobin is also called as _____
Oligochromia
Increased in Hemoglobin is found in:
Polycythemia
Dehydration
Poorly compensated heart diseases with cyanosis
Changing from high to low altitudes
It is the presence of free hemoglobin in the plasma.
Hemoglobinemia
Hemoglobinuria is found in:
Severe infection
Severe burn
Poisoning with potassium chlorate and mushroom
Paroxysmal hemoglobinuria
HTR (Hemolytic Transfusion Reaction)
Principle of Acid Hematin Method:
Hemoglobin is converted to acid with dilute 0.1 N HCl and resulting brownish yellow color produced upon the addition of distilled water with the color standard in the comparator block.
List down the step-by-step procedure in Acid Hematin Method:
- Introduce 0.1 N HCl solution up to mark 2 of the Sahli graduated tube.
- Using the Sahli pipette, draw blood up to 20 ul.
- Dispense the measured volume of the blood into the graduated tube.
- Thoroughly mix with the use of the stirrer and allow to stand for 5 minutes.
- Add distilled water drop by drop, stirring after each addition and compare with the comparator block.
- When the resulting solution compares with color standard in the comparator block, get the reading either from grams or percentage.
Reference Range for Adult Male:
13.0-17.0 gm/dL
Reference Range for Adult Females that are not Pregnant:
12.0-15.0 gm/dL
Reference Range for Adult Females that are pregnant:
11.0-14.0 gm/dL
Reference Range for Children ages 6-12 years:
11.5-15.5 gm/dL
Reference Range for Children aged 6 months to 6 years:
11.0-14.0 gm/dL
Reference Range for Children aged 2 to 6 months:
9.5-14.0 gm/dL
Reference Range at Birth (Full Term):
13.6-19.6 gm/dL
<7 gm/dL indicates that the patient has _____.
Sever Anemia
> 20 g/dL indicates that the patient has _____.
Hyperviscosity
Crticial Values for Severe Anemia:
<7 gm/dL
Critical Values for Hyperviscosity:
> 20 gm/dL
What solution or reagent is used in the Acid Hematin Method?
0.1 N HCl solution
In the procedure, we introduce 0.1 N HCl solution into the Sahli pipette up to what mark?
Mark 2 of the Sahli pipette
Using the Sahli pipette, we draw blood up to what mark?
20 ul
After mixing the HCl solution and blood, we mix it with a stirrer and allow it to stand for how many minutes?
5 minutes
When the resulting solution compares with color standard in the comparator block, we get the reading either from _____ or _____.
Grams
Percentage
