Hematocrit Determination Macro Method Flashcards
“Hematocrit” means _____
“To Separate Blood”
Hematocrit can often be referred as:
Packed Cell Volume
Volume of Erythrocytes
Reading of Packed Cells
A test that measures the proportion of red blood cells to plasma in peripheral blood.
Hematocrit
Hematocrit is part of _____
CBC (Complete Blood Count)
A test that directly measures the RBC Mass.
Hematocrit
The results from Hematocrit testing are expressed as _____
Volume of packed cell in whole blood (PCV)
Hematocrit is an important measurement in the determination of _____ or _____.
Anemia
Polycythemia
Hematocrit is a good simple screening test for _____.
Anemia
True or False:
Hematocrit results are not reliable.
False
Hematocrit results are reliable.
Materials needed for Hematocrit Testing using Macro Method:
Wintrobe Tube
Long Stem Pipette
Centrifuge
Anticoagulated Blood
Length of a Wintrobe Tube:
11.5 cm
Bore of a Wintrobe tube:
2.5 mm
Wintrobe Tube has how many graduations?
Two graduations: 0-100
Ascending and Descending
What is the color of graduations of a Wintrobe Tube used for Macro Hematocrit Test?
White
List down the procedures for Macro Method of Hematocrit Test?
- Mix the blood.
- With the use of long pipette, fill the wintrobe tube to the 10 mark.
- Centrifuge the blood for 30 minutes.
- Read the height of the packed red cell on the scale at the right side of the tube, which is graduated from 0-10 cm from the bottom to top, read upward.
In the procedure for Macro Hematocrit Method, how many minutes is needed for centrifugation of blood?
30 minutes
Graduations of a White Wintrobe Tube from bottom to top:
Ascending or Descending?
Ascending
What color tube stopper is used to store blood for the Macro Hematocrit Test?
Purple/Lavender (EDTA)
Formula for Hematocrit:
(Packed Cell Volume/Total Volume Used which is usually 10) x 100
False Increase of Hematocrit Results:
Undercentrifugation
Prolonged Standing
Inclusion of Buffy Coat
False Decrease of Hematocrit Results:
Overcentrifugation
Hemolyzed Sample
Increased Concentration of an Anticoagulant
Reference Value for Male in Hematocrit:
40% - 54% or 0.40 L/L - 0.54 L/L
Reference Value for Female in Hematocrit:
35% - 49% or 0.35 L/L - 0.49 L/L
