Hematocrit Determination Macro Method

Question 1

“Hematocrit” means _____

Answer 1

“To Separate Blood”

Question 2

Hematocrit can often be referred as:

Answer 2

Packed Cell Volume
Volume of Erythrocytes
Reading of Packed Cells

Question 3

A test that measures the proportion of red blood cells to plasma in peripheral blood.

Answer 3

Hematocrit

Question 4

Hematocrit is part of _____

Answer 4

CBC (Complete Blood Count)

Question 5

A test that directly measures the RBC Mass.

Answer 5

Hematocrit

Question 6

The results from Hematocrit testing are expressed as _____

Answer 6

Volume of packed cell in whole blood (PCV)

Question 7

Hematocrit is an important measurement in the determination of _____ or _____.

Answer 7

Anemia
Polycythemia

Question 8

Hematocrit is a good simple screening test for _____.

Answer 8

Anemia

Question 9

True or False:

Hematocrit results are not reliable.

Answer 9

False

Hematocrit results are reliable.

Question 10

Materials needed for Hematocrit Testing using Macro Method:

Answer 10

Wintrobe Tube
Long Stem Pipette
Centrifuge
Anticoagulated Blood

Question 11

Length of a Wintrobe Tube:

Answer 11

11.5 cm

Question 12

Bore of a Wintrobe tube:

Answer 12

2.5 mm

Question 13

Wintrobe Tube has how many graduations?

Answer 13

Two graduations: 0-100
Ascending and Descending

Question 14

What is the color of graduations of a Wintrobe Tube used for Macro Hematocrit Test?

Answer 14

White

Question 15

List down the procedures for Macro Method of Hematocrit Test?

Answer 15

1. Mix the blood.
2. With the use of long pipette, fill the wintrobe tube to the 10 mark.
3. Centrifuge the blood for 30 minutes.
4. Read the height of the packed red cell on the scale at the right side of the tube, which is graduated from 0-10 cm from the bottom to top, read upward.

Question 16

In the procedure for Macro Hematocrit Method, how many minutes is needed for centrifugation of blood?

Answer 16

30 minutes

Question 17

Graduations of a White Wintrobe Tube from bottom to top:

Ascending or Descending?

Answer 17

Ascending

Question 18

What color tube stopper is used to store blood for the Macro Hematocrit Test?

Answer 18

Purple/Lavender (EDTA)

Question 19

Formula for Hematocrit:

Answer 19

(Packed Cell Volume/Total Volume Used which is usually 10) x 100

Question 20

False Increase of Hematocrit Results:

Answer 20

Undercentrifugation
Prolonged Standing
Inclusion of Buffy Coat

Question 21

False Decrease of Hematocrit Results:

Answer 21

Overcentrifugation
Hemolyzed Sample
Increased Concentration of an Anticoagulant

Question 22

Reference Value for Male in Hematocrit:

Answer 22

40% - 54% or 0.40 L/L - 0.54 L/L

Question 23

Reference Value for Female in Hematocrit:

Answer 23

35% - 49% or 0.35 L/L - 0.49 L/L