Hematocrit Determination Micro Method

Question 1

Materials needed for Micro Hematocrit Testing:

Answer 1

Capillary Tube
Sealing Clay
Microhematocrit Reader
Microhematocrit Centrifuge

Question 2

What color of Capillary Tube is used for Microhematocrit Test?

Answer 2

Capillary Tube with Blue Band

Question 3

Why a capillary tube with red band is not recommended for Microhematocrit Test?

Answer 3

This is because the blood is already stored in an anticoagulated tube (EDTA) and when it is placed in a capillary tube with red band, the anticoagulant would be doubled resulting in false results.

Question 4

When placing the capillary tube with blood sealed with clay and wax, what is the placement in the microhematocrit centrifuge?

Answer 4

The clay ends must facing the outside. The clay must be touching the rubber.

Question 5

How much blood must be filled in the capillary tube for Microhematocrit Test?

Answer 5

3/4 full

Question 6

How do we seal the capillary tube used for Microhematocrit Test?

Answer 6

We seal the part of the capillary tube with color band by holding the tube horizontally, seal with clay, and rotate slightly preferrably a 90 degree angle.

Question 7

Speed and time for centrifugation for Microhematocrit Test?

Answer 7

5 minutes at full speed of 12,000 rpm

Question 8

Sources of error in Microhematocrit Test:

Answer 8

• Incomplete sealing of the microhematocrit tubes - falsely low results
• Inadequate centrifugation
• Time and speed of centrifugation
• Overanticoagulated