Introduction to Hemocytometer

Question 1

Hemo means _____

Answer 1

Blood

Question 2

Cyto means _____

Answer 2

Cell

Question 3

Meter means _____

Answer 3

Measurement

Question 4

An instrument used to count blood cells.

Answer 4

Hemocytometer

Question 5

It is the name given to a thick glass slide.

Answer 5

Neubauer’s Slide

Question 6

In the center of the Neubauer’s Slide, there is an _____.

Answer 6

H-shaped groove

Question 7

Depth of the scales of the Neubauer’s Slide:

Answer 7

1/10mm or 0.1mm

Question 8

Neubauer’s slide with a cover slip over it is called a _____.

Answer 8

Neubauer’s Chamber

Question 9

What is the measurement of the length and the width of a Neubauer’s slide?

Answer 9

3mm wide
3mm long

Question 10

The whole scale of Neubauer’s slide is divided into how many big squares?

Answer 10

9 big squares

Question 11

Measurement of each bigger square in Neubauer’s slide:

Answer 11

1mm long
1mm wide

Question 12

Hemocytometer includes:

Answer 12

Neubauer’s Slide
Cover Slip
RBC Pipette
WBC Pipette

Question 13

1 large square is divided into how many smaller squares?

Answer 13

16 smaller squares

Question 14

What part of the Neubauer’s Chamber is used for WBC Counting?

Answer 14

Four Corner Large Squares

Question 15

What is the total number of Small Squares used for WBC Counting?

Answer 15

64 Small Squares

Question 16

What part of the Neubauer’s Chamber is used for RBC Counting?

Answer 16

Central Square

Question 17

The Central Square is subdivided into how many smaller squares?

Answer 17

25 Smaller Squares

Question 18

Total Number of Large Sqaures used for WBC Counting:

Answer 18

4 Large Squares

Question 19

Total Number of Smallest Squares used for RBC Counting:

Answer 19

80 Smallest Squares

Question 20

Area where platelets are counted?

Answer 20

All of the Small Squares in the Central Square

Question 21

Measurement of 1 whole Large Square:

Answer 21

1mm long
1mm wide

Question 22

Measurement of 1 Small Square:

Answer 22

0.2mm long
0.2mm wide

Question 23

Measurement of 1 Smallest Square:

Answer 23

0.5mm long
0.5mm wide

Question 24

Length of 1 Small Square:

Answer 24

1/4 mm (0.25mm)

Question 25

Width of 1 Small Square:

Answer 25

1/4 mm (0.25mm)

Question 26

Depth of 1 Small Square:

Answer 26

1/10 mm (0.1 mm)

Question 27

Volume of 1 Small Square:

Answer 27

= 1/4 x 1/4 x 1/10 or 0.25 x 0.25 x 0.1
= 1/160 mm^3 or 0.00625 mm^3

Question 28

Length of 1 Smaller Square:

Answer 28

1/5 mm (0.2 mm)

Question 29

Width of 1 Smaller Square:

Answer 29

1/5 mm (0.2 mm)

Question 30

Depth of 1 Smaller Square:

Answer 30

1/10 mm (0.1mm)

Question 31

Volume of 1 Smaller Square:

Answer 31

= 1/5 x 1/5 x 1/10 or 0.2 x 0.2 x 0.1
= 1/250 mm^3 or 0.004 mm^3

Question 32

Length of 1 Smallest Square:

Answer 32

1/20 mm (0.05 mm)

Question 33

Width of 1 Smallest Square:

Answer 33

1/20 mm (0.05 mm)

Question 34

Depth of 1 Smallest Square:

Answer 34

1/10 mm (0.1 mm)

Question 35

Volume of 1 Smallest Square:

Answer 35

= 1/20 x 1/20 x 1/10 or 0.05 x 0.05 x 0.1
= 1/4000 mm^3 or 0.00025 mm^3

Question 36

In counting rules, we do not count the cells touching:

Answer 36

• Bottom Line
• Right Line

Question 37

Color of the bead of an RBC Pipette:

Answer 37

Red Bead

Question 38

Color of the bead on a WBC Pipette:

Answer 38

White Bead

Question 39

Graduation mark of an RBC Pipette:

Answer 39

101

Question 40

Graduation mark of a WBC Pipette:

Answer 40

11

Question 41

Size of the bulb in an RBC Pipette:

Answer 41

Larger

Question 42

Size of the bulb in a WBC Pipette:

Answer 42

Smaller

Question 43

Size of the lumen in an RBC Pipette:

Answer 43

Smaller

Question 44

Size of the lumen in a WBC Pipette:

Answer 44

Larger

Question 45

What is the Objective used on the microscope to see the general formation of slide?

Answer 45

4x

Question 46

What is the Objective used on the microscope for WBC Counting?

Answer 46

10x

Question 47

What is the Objective used on the microscope for RBC Counting?

Answer 47

40x