Introduction to Hemocytometer Flashcards
Hemo means _____
Blood
Cyto means _____
Cell
Meter means _____
Measurement
An instrument used to count blood cells.
Hemocytometer
It is the name given to a thick glass slide.
Neubauer’s Slide
In the center of the Neubauer’s Slide, there is an _____.
H-shaped groove
Depth of the scales of the Neubauer’s Slide:
1/10mm or 0.1mm
Neubauer’s slide with a cover slip over it is called a _____.
Neubauer’s Chamber
What is the measurement of the length and the width of a Neubauer’s slide?
3mm wide
3mm long
The whole scale of Neubauer’s slide is divided into how many big squares?
9 big squares
Measurement of each bigger square in Neubauer’s slide:
1mm long
1mm wide
Hemocytometer includes:
Neubauer’s Slide
Cover Slip
RBC Pipette
WBC Pipette
1 large square is divided into how many smaller squares?
16 smaller squares
What part of the Neubauer’s Chamber is used for WBC Counting?
Four Corner Large Squares
What is the total number of Small Squares used for WBC Counting?
64 Small Squares
What part of the Neubauer’s Chamber is used for RBC Counting?
Central Square
The Central Square is subdivided into how many smaller squares?
25 Smaller Squares
Total Number of Large Sqaures used for WBC Counting:
4 Large Squares
Total Number of Smallest Squares used for RBC Counting:
80 Smallest Squares
Area where platelets are counted?
All of the Small Squares in the Central Square
Measurement of 1 whole Large Square:
1mm long
1mm wide
Measurement of 1 Small Square:
0.2mm long
0.2mm wide
Measurement of 1 Smallest Square:
0.5mm long
0.5mm wide
Length of 1 Small Square:
1/4 mm (0.25mm)
Width of 1 Small Square:
1/4 mm (0.25mm)
Depth of 1 Small Square:
1/10 mm (0.1 mm)
Volume of 1 Small Square:
= 1/4 x 1/4 x 1/10 or 0.25 x 0.25 x 0.1
= 1/160 mm^3 or 0.00625 mm^3
Length of 1 Smaller Square:
1/5 mm (0.2 mm)
Width of 1 Smaller Square:
1/5 mm (0.2 mm)
Depth of 1 Smaller Square:
1/10 mm (0.1mm)
Volume of 1 Smaller Square:
= 1/5 x 1/5 x 1/10 or 0.2 x 0.2 x 0.1
= 1/250 mm^3 or 0.004 mm^3
Length of 1 Smallest Square:
1/20 mm (0.05 mm)
Width of 1 Smallest Square:
1/20 mm (0.05 mm)
Depth of 1 Smallest Square:
1/10 mm (0.1 mm)
Volume of 1 Smallest Square:
= 1/20 x 1/20 x 1/10 or 0.05 x 0.05 x 0.1
= 1/4000 mm^3 or 0.00025 mm^3
In counting rules, we do not count the cells touching:
- Bottom Line
- Right Line
Color of the bead of an RBC Pipette:
Red Bead
Color of the bead on a WBC Pipette:
White Bead
Graduation mark of an RBC Pipette:
101
Graduation mark of a WBC Pipette:
11
Size of the bulb in an RBC Pipette:
Larger
Size of the bulb in a WBC Pipette:
Smaller
Size of the lumen in an RBC Pipette:
Smaller
Size of the lumen in a WBC Pipette:
Larger
What is the Objective used on the microscope to see the general formation of slide?
4x
What is the Objective used on the microscope for WBC Counting?
10x
What is the Objective used on the microscope for RBC Counting?
40x
