signal transduction Flashcards

Dr Danny Zisterer

1
Q

Communication by extracellular signals(so racist that David thought racist thoughts)

A

Synthesis
* Release of signalling molecule by signalling cell
* Transport of signal to target cell
* Detection of signal by specific receptor protein
* Transduction of signal (e.g. using second messengers)
* Response: change in cellular metabolism, function or
development triggered by receptor:signal complex
* Termination of signal

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2
Q

name the four types of cell surface receptors

A

G-protein coupled receptors (GPCR),
Ion-channel receptors,
Tyrosine kinase-linked receptors,
Receptors with intrinsic enzymatic activity.

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3
Q

example of g protein receptors

A

epinephrine

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4
Q

example of Ion-channel receptors

A

acetylcholine

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5
Q

example of Tyrosine kinase-linked receptors

A

erythropoietin

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6
Q

Receptors with intrinsic enzymatic activity

A

insulin

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7
Q

Lipophilic hormones with intracellular receptors

A

steroid hormones, thyroxine, Vitamin D

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8
Q

Lipophilic hormones with cell-surface receptors

A

prostaglandins

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9
Q

Hydrophilic hormones with cell surface receptors

A

Peptide hormones e.g. insulin and glucagon
Small charged molecules e.g. epinephrine and histamine

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10
Q

An example of a Signal Transduction Pathways

A

epinephrine+beta adergentic receptor = energy store mobalistaion

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11
Q

Common second messengers

A

cAMP, IP3, Calcium ion

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12
Q

what is the problem associated with secondary messengers?

A

Use of common second messengers in multiple
signalling pathways creates both opportunities (cross
talk) and potential problems

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13
Q

Protein phosphorylation is a common means of
information transfer but how? ie name the enzyme.

A

Specific enzymes, known as protein kinases
phosphorylate target proteins. ATP is the most common donor

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14
Q

what do protein kinases do?

A

transfer phosphoryl groups from ATP to specific serine, threonine
and tyrosine residues on specific proteins

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15
Q

what removes the phosphate groups hydrolytically?

A

Protein phosphatases

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16
Q

*Phosphorylation and dephosphorylation reactions are not
the reverse of one another.Why is this so?

A

Each is essentially irreversible
under physiological conditions.

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17
Q

Indeed a phosphate group does what?

A

A phosphate group adds two negative charges to the
protein-allows new electrostatic interactions to be formed. A phosphate group can allow for three hydrogen binds to be formed.

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18
Q

what is amplification?

A

when enzymes activate enzymes, the number of affected molecules increases geometrically in an enzyme cascade.

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19
Q

Cyclic AMP activates protein kinase A (PKA)
by what?

A

altering the quaternary structure

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20
Q

g-protein receptors look like what?

A

contains seven helices that
span the membrane bilayer

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21
Q

how are G-Protiens activated by ligands?

A

Cytoplasmic loops and C-termini change
conformation in response to ligand
binding

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22
Q

name some of the Biological Functions Mediated by
GPCR or 7TM Receptor

A

Smell
Taste
Neurotransmission
Hormone Action
Hormone Secretion
Control of Blood Pressure
Embryogenesis
Development
Vision
Viral Infection

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23
Q

what was the first cellular g-protein receptor identified

A

Rhodopsin: takes part in visual signal
transduction

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24
Q

GCPR are the sites for what?

A

Target of almost half of all modern pharmaceutical
drugs (e.g. beta-blockers, anti-histamines and
various psychiatric drugs)

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25
Q

in the heteromeric structure of G-proteins what transformation happens when binding

A

Once GTP binds to a subunit it changes
conformation and dissociates from betay
dimer

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26
Q

which is active and inactive G coupled protein forms?

A

GDP-bound (inactive)
GTP-bound (active)

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27
Q

Effector enzymes

A

adenylate cyclase

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28
Q

b-Adrenergic receptor signalling pathway

A

begins with epinephrin binding to b-adrenergic receptor. GP is phosphorylase then the alpha subunit becomes dislodged and binds to adenylate cyclase. then to cyclic amp with is a secondary receptor.

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29
Q

HOW IS SIGNAL TRANSDUCTION BY THE GPCR
TERMINATED?

A

Dissociation of signalling molecule from receptor &
Phosphorylation of cytoplasmic C-terminus of receptor and
subsequent binding of b-arrestin (desensitisation)

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30
Q

why is epinephrine needed in times of stress?

A

epinephrine produces food as a result of epinephrine/adrenaline
binding to hepatic and adipose cells respectively (b-adrenergic
receptor)
Also,Epinephrine also binds to b-adrenergic receptors on heart increasing
contraction rate which increases blood supply to tissues

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31
Q

when will receptor dissociate?

A

when levels of ephinephrine go below kd.

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32
Q

why are so many signals amplified by camp?

A

because some tissues inhibit amp for a response whilst others stimulate it .

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33
Q

A kinase anchoring proteins

A

AKAPs are multivalent adaptor proteins: one part binds to R
subunits of PKA and another to the specific structure within the cell
(e.g. microtubules)-confines the PKA to the vicinity of that structure.

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34
Q

PHOSPHOINOSITIDE CASCADE

A

ligand > receptor> activated g protien> activated phospholipase C>cleavage of phospholipid pip2 and ip3 and DAG.

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35
Q

pip 2 is cleaved into

A

DAG and IP3

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36
Q

The enzyme phospholipase C does what?

A

The enzyme phospholipase C cleaves PIP2 yielding IP3 and DAG

37
Q

How are the IP3
- and DAG-initiated signals
turned off

A

IP3
is rapidly metabolised to inositol which
cannot open Ca2+ channel
DAG may be (1) phosphorylated to
phosphatidate or (2) hydrolysed to glycerol and
fatty acids

38
Q

Calcium ion is a widely used second messenger how?

A

Calcium binds tightly to proteins and induces
conformational changes

39
Q

what does calmodulin act as?

A

Calmodulin acts as a calcium sensor in nearly all
eukaryotic cells.

40
Q

When cytosolic Ca2+ level is raised above what? ,
calmodulin is activated

41
Q

calmodulin has how many binding sites?

A

4 binding
sites also think of your hand

42
Q

After binding what surfaces get exposed

A

hydrophobic

43
Q

calmodiun targets what

A

particular a-helix (in
purple) in CaM kinase I

44
Q

what signalling pathways get kicked off by tyrosine kinase receptors (2)

A

Insulin signalling pathway
*EGF signalling pathway

45
Q

Insulin receptor

A

is a dimer of two identical units (one a
subunit and one b subunit linked by a disulfide bond
Each b-subunit contains a protein
kinase domain

46
Q

how do you activate this receptor?

A

On binding of insulin, the cytoplasmic
domain of the receptor which is a
tyrosine kinase, becomes autophosphorylated on tyrosine OH
groups resulting in activation of the
insulin receptor.

47
Q

the activation of insulin receptor

A

Binding of insulin to its receptor results in a conformational change which
allows each b-subunit phosphorylate three key tyrosine residues on the other bsubunit. Phosphorylation of these three tyrosine residues in the activation loop
(in red) of the kinase domain of the insulin receptor cause the loop to swing
across the structure

48
Q

what does akt 1 do?

A

Akt-1 moves through the cell phosphorylating target proteins (e.g. enzymes that
stimulate glycogen synthesis and components important in trafficking the glucose
transporter GLUT4 to the cell surface)

49
Q

insulin increases the rate of glucose by how much?

50
Q

glucose transporters are stored where?

A

in vesicles

51
Q

How are insulin receptor substrates (IRS)
recruited to the activated insulin
receptor?

A

The IRS attaches by its conserved SH2
domain to the phosphorylated receptor
and it then becomes phosphorylated on
tyrosine residues

52
Q

there are 3 stages where amplification can occur when?

A

at enzymic reaction points

53
Q

how is insulin signalling terminated?

A

1.Protein tyrosine phosphatases remove phosphoryl groups from
insulin receptor
2.Lipid phosphatases remove the phosphoryl groups from inositol
lipids (PIP3
to PIP2)
3.Protein serine phosphatases remove phosphoryl group from
activated protein kinases e.g. Akt

54
Q

what is unique about the EGF receptor

A

The EGF receptor is a dimer of identical subunits but, unlike the
insulin receptor, the units exists as monomers until EGF
binds. EGF so the dimer binds two
molecules of ligand

55
Q

what is the structure of EGF receptor?

A

EFG binding domain, transmembrane helix, kinase domain, c terminus tail.

56
Q

what does the binding of EGF do?

A

EGF binding induces a conformational change allowing the
dimerisation arm to extend from each receptor molecule.

57
Q

what is the small G protien that is activated in the EGF pathway

58
Q

EGF signalling leads to activation of a small G
protein Ras outline this pathway.

A

1.Binding of EGF causes dimerisation and
phosphorylation of tyrosine residues on C-terminal tail
of receptor
An adapter protein, GRB2, binds to phosphotyrosines
on activated RTK
GRB2 binds to Sos protein (a guanine nucleotideexchange factor or GEF)
Sos promotes exchange of GDP-Ras (inactive) to
GTP-Ras (active)
Ras activates downstream signalling pathways

59
Q

on the GRB 2 protein SH2 binds to

A

The SH2 domain binds to
phosphotyrosine residues

60
Q

SH3 domain

A

proline-rich regions
on other proteins

61
Q

How is the EGF signalling pathway terminated?

A

Phosphatases remove phosphoryl groups from tyrosine residues
on EGF receptor and from serine, threonine and tyrosine
residues in the protein kinases that participate in signalling
cascade
Ras possesses intrinsic GTPase activity. This is accelerated by
GAPs (GTPase-activating proteins) which facilitate GTP
hydrolysis

62
Q

main changes for drosophila

A

EFG> BOSS
EGF Receptor>sev
GRB2 > Drk

63
Q

Most common cancer gene in the human population

A

Ras Gene
Most common mutation leads to loss of ability of Ras
protein to hydrolyse GTP

64
Q

constitutively active tyrosine kinase

A

Results in fusion protein known as BCR-ABL
also- Translocation of genetic material between chromosomes
9 and 22 causes the c-abl gene (9) to be inserted into bcr
gene (22)

65
Q

leukaemia or (CML) have what?

A

CML cells express a unique target for anticancer agents .Gleevec is a tyrosine kinase inhibitor (TKI)- it inhibits
BCR-ABL

66
Q

what happens in the Her-2 gene?

A

The Her-2 receptor can signal even in the absence of ligand-it
adopts an extended dimerization arm and is thus constitutively
active.

67
Q

Herceptin

A

enhanced receptor degradation or indeed, antibody-dependent
cellular cytotoxicity

68
Q

Cetuximab

A

This antibody inhibits the EGFR by competing with
EGF for binding to receptor. The antibody also sterically blocks
the dimerisation arm preventing it from extending

69
Q

Notch signalling pathway-

A

juxtaposed ligands and receptors.

70
Q

what does the notch signalling do?

A

It regulates cellular identity, proliferation, differentiation and
apoptosis
apoptosis is programmed cell death.

71
Q

what is lateral inhibition

A

lateral inhibiton is adjacent and developmentally
equivalent cells assume completely different fates

72
Q

Basic operation of the Notch pathway

A

Key players:
▪ Delta-type ligand
▪ Receptor Notch
▪ Proteases
▪ CSL transcription factor

73
Q

where is the notch signal receptor formed?

A

The Golgi then it is transported to cell surface.

74
Q

what are the notch target genes

A

HES, CYCLIN D1 and C-MYC

75
Q

How is Notch Signalling Pathway Switched Off?

A

An E3 ubiquitin ligase attaches multiple ubiquitin molecules to
Notch-ICD thus targeting it to the proteasome
* The proteasome is a large multifunctional protease complex in
the cytosol that degrades intracellular proteins marked for
destruction by ubiquitin

76
Q

Two major classes of Notch inhibitors:

A

Y-secrase and monochrome antibody’s

77
Q

Protein synthesis can be globally regulated by

A

TOR Kinase

78
Q

TOR kinase regulates what?

A

translation, transcription

79
Q

what are the mamalian TOR kinase holders two different types

A

mTORC1 and mTORC2

80
Q

what are the other two components for mtorc1

A

receptor and mLST8

81
Q

Active mTORC1 controls
protein synthesis by
phosphorylating two key
proteins

A

S6K1/2 and 4EBP1

82
Q

S6K1/2

A

phosphorylates
ribosomal proteins
leading to an
increase in the rate
of protein synthesis

83
Q

4E-BP1

A

inhibits interaction of a
key translation initiation factor
with mRNA so inhibits protein
synthesis. When phosphorylated by mTORC1, 4E-BP1 releases the initiation factor thus stimulating translation initiation

84
Q

an antagonistic relationship between
the mTOR and AMPK pathways exists explain this

A

work opposingly

84
Q

when is AMPK activated?

A

decrease in energy level, nutrient starvation or increase in the
AMP/ATP ratio

85
Q

what are the AMPK similar in plants

86
Q

SnRK1

A

SnRK1 is a central integrator of stress and energy modulating the expression of
more than 1000 genes through phosphorylation of various transcription factors

87
Q

how is plant snrk1 regulated ie. what sugars are responsible?

A

glucose6-phosphate (G6P) and trelahose-6-phosphate (T6P)