Sickle Scalers - Plaque and Biofilm Flashcards
- a naturally acquired bacterial biofilm that develops on teeth
- Multi-species biofilm
- Sticky, colorless film which constantly forms over teeth
- Removed with mechanical force such as toothbrushing/flossing)
Dental plaque
- Hard calcified deposit of plaque that has become mineralized
- Not easily removed with homecare tools
Calculus
Are the following mineralized or non-mineralized? •Dental plaques •Acquired pellicle •Plaque biofilm •Materia alba •Food particles
Non-mineralized
Are the following mineralized or non-mineralized?
•Calculus
Mineralized
Phases of ______ Accumulation
- Formation of pellicle on tooth surface
- Initial adhesion/attachment of bacteria
- Colonization/plaque maturation
Dental Plaque
- Originates from the saliva
- Thin, structureless membrane; coats all surfaces in the oral cavity within 1 minute of being removed
- Acellular in nature; consists primarily of glycoproteins
- Colonization of acquired pellicle serves as a nutrient for bacteria
- Takes approximately 7 days for it to develop into its condensed, mature structure
Acquired pellicle
- Protective
- Provides a barrier against acids, thus reducing dental caries attack
- Lubrication
- Keeps surfaces moist, prevents drying
- Nidus for bacteria
- Key role in development of plaque
- Aides in the attachment of calculus
Pellicle
•Dense, non-calcified, organized mass of bacterial colonies enclosed in a gel-like intercellular matrix
Plaque Biofilm
_____ colonizers: gram (+) aerobic and facultative organisms (actinomyces, streptococcus) attached to pellicle
•Approximately 47-85% cocci during first 4 hours
•Most abundant colonization occurs on proximal surfaces, fissures and gingival sulcus
•Initial colonizers
_____ colonizers: Pi, Pg, Capnocytophaga species, spirochetes, motile rods, gram (-) anaerobic organisms •These colonizers are the etiologic initiators of caries and periodontitis
•Secondary colonizers
Day ____ of biofilm formation:
gram (+) cocci
1
Day ____ - ____ of biofilm formation:
filamentous forms grow on cocci; intercellular matrix forms and connects colonies
2-4
Day ____ - ____ of biofilm formation:
filamentous forms increase; rods and fusobacteria appear
4-7
Day ____ - ____ of biofilm formation:
vibrios and spirochetes appear; gram (-) species increase; clinical inflammation visible
7-14
Day ____ - ____ of biofilm formation:
gingivitis clinically evident
•Plaque is composed of densely packed vibrios, spirochetes and filamentous bacteria
•Biofilm is well-established; channels established to distribute nutrients, remove wastes, allow free-flowing bacteria to form new colonies
14-24
- Matrix that surrounds the bacteria within the plaque biofilm
- Composed of inorganic and organic components that originate from the bacteria
- Major components: polysaccharides derived from bacterial metabolism of CHO
- Minor components: salivary glycoproteins
Intercellular Plaque Matrix