Serum Dilution Flashcards
What is Serum dilution?
It is a laboratory technique used to reduce the concentration of substances present in the serum, the liquid portion of blood obtained after coagulation.
Serum Dilution
It involves adding a known volume of a diluent, typically a buffered saline solution, to a smaller volume of serum, resulting in a…
lower concentration of analytes.
Serum dilution is essential in various medical and research applications… why?
It can accurately measure and analyze specific components in the blood.
In medical diagnostics, serum dilution is frequently employed to quantify substances present in the blood at extremely high concentrations.
These substances may include…
hormones, enzymes, proteins, electrolytes, and various other analytes.
When certain components are present in excess, they can saturate the measurement assays, leading to…
By diluting…
Leads to inaccurate results.
By diluting the serum, the concentration of the target analyte falls within the linear range of the assay, ensuring accurate and reliable measurements.
Researchers also use ______techniques to investigate specific blood components and study their behavior under various conditions.
______enable researchers to explore a broader range of concentrations and observe how different factors may influence the analyte of interest.
serum dilution
Dilutions
The serum dilution process involves precise measurements and calculations to ensure accurate dilution ratios.
Careful attention must be given to maintaining the dilution accuracy, as inaccuracies can significantly impact test results and lead to…
misinterpretation of the data.
In this laboratory procedure, the diluent is added in incremental steps, and each step is mixed thoroughly to achieve homogeneity before further dilution.
The final diluted serum can be tested using various laboratory techniques, such as (3), to measure the concentration of the target analyte accurately.
immunoassays
spectrophotometry
chromatography
is a crucial technique used in clinical laboratories and research settings to reduce the concentration of substances in the blood serum for accurate measurement and analysis.
serum dilution
By carefully diluting the serum, healthcare professionals and researchers can…
obtain reliable data and make informed decisions in diagnosing and studying various medical conditions and biochemical processes.
Serum is sometimes diluted in saline or other diluents to determine its relative antibody concentration.
It is customary to express the dilution as 1 part of_____ contained in the total number of parts of the dilution.
For example, to test the serum at one-tenth its original concentration, a dilution of 1 part in_ may be made by mixing 1 mL of serum with____ of saline.
The final volume is 10, and the dilution is expressed as a 1-in-1____. The diluted material contains one-tenth (1/10 or 0.1) of the unmodified serum. It is often customary to report the titer of an antibody as the reciprocal of the highest dilution that retains a 1+ agglutination. Therefore, serum that reacts at a dilution of 1/32 is considered to have a titer of 32
serum
dilution
10
9 mL
is a process to reduce the strength of a concentrated solution
DILUTION
TWO ENTITIES: the____, which is the material being diluted, and______, which is the medium making up the rest of the solution.
SOLUTE
DILUENT(or SOLVENT)
= Volume of Solute + Volume of Diluent
Total Volume of the Solution
= amount of the sample or (analyte)
Volume of Solute