HBsAg TEST

Question 1

Principle

Answer 1

Immunochromatographic Assay

Question 2

The membrane strip of the test device is pre-coated with_______ on the______ region.

Answer 2

mouse monoclonal anti-HBs

test (T) band

Question 3

The__________ and the specimen move along on the membrane
_______to the _____ region and forms a visible line as the antibody-antigen-antibody gold particle complex forms.

Answer 3

mouse monoclonal anti-HBs-colloid gold conjugate

chromatographically

test region (T)

Question 4

• The test strip also contains_______, which moves across the membrane and reaches the ________region.

Answer 4

chicken IgY conjugate gold colloid

control (C) band region

Question 5

In the control band region, the________ interacts with immobilized_______, leading to the appearance of a______-colored band.

This serves as a control for the proper functioning of the test, ensuring that enough volume of the specimen was added, and that the reagents used in the test are functioning correctly.

Answer 5

chicken IgY conjugate

mouse monoclonal Chicken IgY antibodies

purple

Question 6

Specimen:

Answer 6

Plasma or Serum

Question 7

Storage:
• If not tested immediately, samples should be refrigerated at_____

• For storage longer than 3 days, freezing (_____) is recommended.
Samples should be brought to ______prior to use.

Answer 7

2-8°C

-20°C

15-30°C

Question 8

Reagents:

Answer 8

Bioline HBsAg test kit

Question 9

Procedure:
1. Ensure all components of the kit and the specimen are at_____ temperature before proceeding
2. Take the test device out of the foil pouch and place it on a dry surface .
3. Label the test device with the patient’s name.
4. Add____ of the specimen into the sample well.
5. As the test commences, you will observe a____ color moving across the result window in the center of the test device.
6. Interpret the test results precisely at_____ minutes.

Answer 9

room

100 uL

purple

20

Question 10

Interpretation of Results

: The presence of only one band (“C” band) within the results window.

Answer 10

Negative

Question 11

: The presence of two color bands (“T” and “C” band) within the results window no matter which band appear first indicates a positive result.

Answer 11

Positive

Question 12

: After performing the test and no purple color band (“C” band) is visible within the results window.

Answer 12

Invalid