Dengue Flashcards
Also known as
Breakbone fever
A mosquito-borne infection caused by a
…
Flavivirus
Transmitted by (2)mosquitoes.
Aedes aegypti and Aedes
albopictus
• Characterized by….
Severe forms of the disease, (2) syndrome, principally affect children.
fever
severe headache
muscle and joint pain
nausea and vomiting
eye pain
rash
dengue hemorrhagic fever, and dengue shock
• Dengue is endemic in more than____ countries, especially in the Philippines.
100
Size and markings of Aedis aegypti
• Black, small to medium in size.
• Lyre-shaped silver markings on the mesonotum.
Aedis aegypti leg marking
• Leg markings:
• Fore- and mid-legs:
• Hind legs:
Narrow white bands at the base of the tarsi.
Five broad white bands, leading to the name “tiger mosquito.”
Aedis aegypti
• Breeding Sites (_____dengue):
• Prefers____ water.
• Found in indoor and outdoor containers:
Examples:
Urban
clear
Old tires, vases, jars, bottles.
Aedes albopictus
• Identification:
Single, longitudinal silvery stripe on the mesonotum.
Aedes albopictus
• Breeding Sites (_____dengue):
• Prefers_____ water.
• Found in indoor and outdoor
containers:
• Examples:_____
Rural
clear
Bamboo stumps, empty coconut shells, artificial containers, tree holes.
Causative agent
Dengue virus
VECTORS OF DENGUE IN THE PHILIPPINES
• Aedes albopictus
• Aedes aegypti
DENGUE VIRUS
• A single-stranded RNA virus of the… family
Flaviviridae family
T or F
• Widely present in tropical and subtropical
regions.
True
T or F
• High morbidity and mortality, making it a major arthropod-borne viral disease.
T
• Serotypes.
• Four main serotypes: (4)
• ______ was detected in____ (October 2013).
DENV-1, DENV-2, DENV-3, DENV-4
DENV-5 ; Malaysia
Genomic Structure:
• Dengue virus genome translates into a single polyprotein encoding:
• 3 structural proteins:
Capsid (C)
pre-membrane (prM/M)
envelope (E)
Genomic Structure:
• Dengue virus genome translates into a single polyprotein encoding:
• 7 nonstructural proteins:
NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5.
• Found at high concentrations in sera of
infected patients during the early clinical phase.
NS1 ANTIGEN
A highly-conserved glycoprotein secreted into the blood during dengue infection.
NS1 ANTIGEN
• Detectable in primary or secondary infections from _____days after fever onset.
NS1: 1-9 days
Positive NS1 test indicates what????
confirms dengue infection but does not identity the serotype
IgM
• Primary dengue infection: Detectable
_______ after illness onset
• Secondary dengue infection: Detectable ______after illness onset
5-10 days
4-5 days
is immunochromatographic assay designed to detect both dengue virus NS1 antigen and IgG/IgM antibodies against dengue virus in human whole blood, serum or plasma.
Bioline™ Dengue Duo
Bioline™ Dengue Duo is ________designed to detect both dengue virus NS1 antigen and IgG/IgM antibodies against dengue virus in human whole blood, serum or plasma.
immunochromatographic assay
Bioline dengue duo
• Ideal diagnostic tool to cover all clinical stages from _____ to _____
• Presumptive differentiation between _______ and _____dengue infections
acute phase to convalescence phase
primary & secondary
Dengue duo
• Easy to use rapid test (Test result:______)
• Shelf life and storage temperature:_____ from the date of manufacturing at_____ temp
15-20 minutes
24 months
1-30 °C
• Purpose: Detects dengue virus NS1 antigen qualitatively.
DENGUE DUO TEST NS1 ANTIGEN
NS1 ANTIGEN
• NS1 antigen in the specimen binds to_______ antibodies conjugated with______
• The antigen-antibody complex migrates via_______.
• Captured by immobilized antibodies, forming a colored Test Line (T)
mouse monoclonal anti-dengue antibodies conjugated with colloidal gold.
chromatography
NS1 antigen
• A_____ ensures the test is functioning properly and should always appear.
Control Line (“C”)
NS1
• Result Interpretation:
Positive:
Negative:
Both “C” (Control Line) and “T” (Test Line) are visible.
Only “C” Line appears.
Purpose: Qualitative detection of IgG and IgM antibodies to dengue virus.
IgG/IgM
IgG/IgM
Purpose: Qualitative detection of IgG and IgM antibodies to dengue virus.
• Detects antibodies for all______ using recombinant envelope proteins.
four dengue virus serotypes
IgG/ IgM
Principle:
• Anti-dengue IgG/IgM in the specimen reacts with…
• The antigen-antibody complex migrates through____.
recombinant envelope
proteins conjugated with colloidal gold
chromatography
IgG/ IgM
• Captured by immobilized anti-human IgG and/or anti-human IgM, forming:
“G” Line: Indicates presence of…
“M” Line: Indicates presence of…
A _______ensures the test is performed correctly and should always appear
Dengue IgG antibodies
Dengue IgM antibodies
Control Line (“C”)
