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Immuno-Sero LAB > Dengue > Flashcards

Dengue Flashcards

1
Q

Also known as

A

Breakbone fever

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2
Q

A mosquito-borne infection caused by a
…

A

Flavivirus

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3
Q

Transmitted by (2)mosquitoes.

A

Aedes aegypti and Aedes
albopictus

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4
Q

• Characterized by….

Severe forms of the disease, (2) syndrome, principally affect children.

A

fever
severe headache
muscle and joint pain
nausea and vomiting
eye pain
rash

dengue hemorrhagic fever, and dengue shock

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5
Q

• Dengue is endemic in more than____ countries, especially in the Philippines.

A

100

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6
Q

Size and markings of Aedis aegypti

A

• Black, small to medium in size.
• Lyre-shaped silver markings on the mesonotum.

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7
Q

Aedis aegypti leg marking

• Leg markings:

• Fore- and mid-legs:
• Hind legs:

A

Narrow white bands at the base of the tarsi.

Five broad white bands, leading to the name “tiger mosquito.”

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8
Q

Aedis aegypti

• Breeding Sites (_____dengue):
• Prefers____ water.
• Found in indoor and outdoor containers:
Examples:

A

Urban

clear

Old tires, vases, jars, bottles.

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9
Q

Aedes albopictus

• Identification:

A

Single, longitudinal silvery stripe on the mesonotum.

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10
Q

Aedes albopictus

• Breeding Sites (_____dengue):
• Prefers_____ water.
• Found in indoor and outdoor
containers:
• Examples:_____

A

Rural

clear

Bamboo stumps, empty coconut shells, artificial containers, tree holes.

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11
Q

Causative agent

A

Dengue virus

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12
Q

VECTORS OF DENGUE IN THE PHILIPPINES

A

• Aedes albopictus

• Aedes aegypti

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13
Q

DENGUE VIRUS
• A single-stranded RNA virus of the… family

A

Flaviviridae family

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14
Q

T or F

• Widely present in tropical and subtropical
regions.

A

True

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15
Q

T or F

• High morbidity and mortality, making it a major arthropod-borne viral disease.

A

T

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16
Q

• Serotypes.
• Four main serotypes: (4)
• ______ was detected in____ (October 2013).

A

DENV-1, DENV-2, DENV-3, DENV-4

DENV-5 ; Malaysia

17
Q

Genomic Structure:
• Dengue virus genome translates into a single polyprotein encoding:
• 3 structural proteins:

A

Capsid (C)
pre-membrane (prM/M)
envelope (E)

18
Q

Genomic Structure:
• Dengue virus genome translates into a single polyprotein encoding:

• 7 nonstructural proteins:

A

NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5.

19
Q

• Found at high concentrations in sera of
infected patients during the early clinical phase.

A

NS1 ANTIGEN

20
Q

A highly-conserved glycoprotein secreted into the blood during dengue infection.

A

NS1 ANTIGEN

21
Q

• Detectable in primary or secondary infections from _____days after fever onset.

A

NS1: 1-9 days

22
Q

Positive NS1 test indicates what????

A

confirms dengue infection but does not identity the serotype

23
Q

IgM
• Primary dengue infection: Detectable
_______ after illness onset
• Secondary dengue infection: Detectable ______after illness onset

A

5-10 days

4-5 days

24
Q

is immunochromatographic assay designed to detect both dengue virus NS1 antigen and IgG/IgM antibodies against dengue virus in human whole blood, serum or plasma.

A

Bioline™ Dengue Duo

25
Q

Bioline™ Dengue Duo is ________designed to detect both dengue virus NS1 antigen and IgG/IgM antibodies against dengue virus in human whole blood, serum or plasma.

A

immunochromatographic assay

26
Q

Bioline dengue duo

• Ideal diagnostic tool to cover all clinical stages from _____ to _____

• Presumptive differentiation between _______ and _____dengue infections

A

acute phase to convalescence phase

primary & secondary

27
Q

Dengue duo

• Easy to use rapid test (Test result:______)
• Shelf life and storage temperature:_____ from the date of manufacturing at_____ temp

A

15-20 minutes

24 months

1-30 °C

28
Q

• Purpose: Detects dengue virus NS1 antigen qualitatively.

A

DENGUE DUO TEST NS1 ANTIGEN

29
Q

NS1 ANTIGEN

• NS1 antigen in the specimen binds to_______ antibodies conjugated with______

• The antigen-antibody complex migrates via_______.

• Captured by immobilized antibodies, forming a colored Test Line (T)

A

mouse monoclonal anti-dengue antibodies conjugated with colloidal gold.

chromatography

30
Q

NS1 antigen

• A_____ ensures the test is functioning properly and should always appear.

A

Control Line (“C”)

31
Q

NS1

• Result Interpretation:
Positive:
Negative:

A

Both “C” (Control Line) and “T” (Test Line) are visible.

Only “C” Line appears.

32
Q

Purpose: Qualitative detection of IgG and IgM antibodies to dengue virus.

A

IgG/IgM

33
Q

IgG/IgM
Purpose: Qualitative detection of IgG and IgM antibodies to dengue virus.
• Detects antibodies for all______ using recombinant envelope proteins.

A

four dengue virus serotypes

34
Q

IgG/ IgM

Principle:
• Anti-dengue IgG/IgM in the specimen reacts with…
• The antigen-antibody complex migrates through____.

A

recombinant envelope
proteins conjugated with colloidal gold

chromatography

35
Q

IgG/ IgM

• Captured by immobilized anti-human IgG and/or anti-human IgM, forming:
“G” Line: Indicates presence of…
“M” Line: Indicates presence of…

A _______ensures the test is performed correctly and should always appear

A

Dengue IgG antibodies

Dengue IgM antibodies

Control Line (“C”)

Immuno-Sero LAB (16 decks)

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